biostudies-literatureBin Mohamed Suffian IFBiotechnology and Biological Sciences Research Council43160https://www.ebi.ac.uk/biostudies/studies/S-EPMC5335696biostudies-literatureUnknown7An E. coli expression system offers a mean for rapid, high yield and economical production of Hepatitis B Virus core (HBc) particles. However, high-level production of HBc particles in bacteria is demanding and optimisation of HBc particle yield from E. coli is required to improve laboratory-scale productivity for further drug delivery applications. Production steps involve bacterial culture, protein isolation, denaturation, purification and finally protein assembly. In this study, we describe a modified E. coli based method for purifying HBc particles and compare the results with those obtained using a conventional purification method. HBc particle morphology was confirmed by Atomic Force Microscopy (AFM). Protein specificity and secondary structure were confirmed by Western Blot and CircScientific reportsYield Optimisation of Hepatitis B Virus Core Particles in E. coli Expression System for Drug Delivery Applications.PMC5335696BB/J008656/1Brown PBui TNishimura YGarcia-Maya MPalermo ARBin Mohamed Suffian IFAl-Jamal KTKondo AOgino CfalseYield Optimisation of Hepatitis B Virus Core Particles in E. coli Expression System for Drug Delivery Applications.An E. coli expression system offers a mean for rapid, high yield and economical production of Hepatitis B Virus core (HBc) particles. However, high-level production of HBc particles in bacteria is demanding and optimisation of HBc particle yield from E. coli is required to improve laboratory-scale productivity for further drug delivery applications. Production steps involve bacterial culture, protein isolation, denaturation, purification and finally protein assembly. In this study, we describe a modified E. coli based method for purifying HBc particles and compare the results with those obtained using a conventional purification method. HBc particle morphology was confirmed by Atomic Force Microscopy (AFM). Protein specificity and secondary structure were confirmed by Western Blot and Circ2017-01-01T00:00:00Z2017 Mar2026-05-30T04:21:34.351Z2019-03-27T02:37:46ZS-EPMC53356962825659210.1038/srep43160