biostudies-literatureUnknown8Suyal DCNitrogen (N) deficiency and low temperature conditions are the prominent facet of Western Himalayan agro-ecosystems. A slight change in the environment alters the protein expression of the microorganisms. Therefore, proteomes of the two psychrotrophs <i>Dyadobacter psychrophilus</i> B2 and <i>Pseudomonas jessenii</i> MP1 were analyzed using two dimensional electrophoresis and MALDI-TOF-MS, to determine the physiological response of altitudinally different but indigenous microorganisms in response to cold stress under N depleting conditions. Functional assessment of 150 differentially expressed proteins from both the psychrotrophs revealed several mechanisms might be involved in cold stress adaptation, protein synthesis/modifications, energy metabolism, cell growth/maintenance, etc. In bothFrontiers in microbiology430https://www.ebi.ac.uk/biostudies/studies/S-EPMC5348510biostudies-literatureCold Stress and Nitrogen Deficiency Affected Protein Expression of Psychrotrophic <i>Dyadobacter psychrophilus</i> B2 and <i>Pseudomonas jessenii</i> MP1.PMC5348510Suyal DCGoel RShouche YYadav AKumar SfalseCold Stress and Nitrogen Deficiency Affected Protein Expression of Psychrotrophic <i>Dyadobacter psychrophilus</i> B2 and <i>Pseudomonas jessenii</i> MP1.Nitrogen (N) deficiency and low temperature conditions are the prominent facet of Western Himalayan agro-ecosystems. A slight change in the environment alters the protein expression of the microorganisms. Therefore, proteomes of the two psychrotrophs <i>Dyadobacter psychrophilus</i> B2 and <i>Pseudomonas jessenii</i> MP1 were analyzed using two dimensional electrophoresis and MALDI-TOF-MS, to determine the physiological response of altitudinally different but indigenous microorganisms in response to cold stress under N depleting conditions. Functional assessment of 150 differentially expressed proteins from both the psychrotrophs revealed several mechanisms might be involved in cold stress adaptation, protein synthesis/modifications, energy metabolism, cell growth/maintenance, etc. In both2017-01-01T00:00:00Z20172025-04-26T01:09:22.736Z2019-03-27T02:38:34ZS-EPMC53485102835226310.3389/fmicb.2017.00430