biostudies-literatureUnknown7(52)Gagne JPWerner syndrome (WS) is characterized by the premature onset of several age-associated pathologies including cancer. The protein defective in WS patients (WRN) is a helicase/exonuclease involved in DNA replication and repair. Here, we present the results of a large-scale proteome analysis that has been undertaken to determine protein partners of different polymorphic WRN proteins found with relatively high prevalence in the human population. We expressed different fluorescently tagged-WRN (eYFP-WRN) variants in human 293 embryonic kidney cells (HEK293) and used a combination of affinity-purification and mass spectrometry to identify different compositions of WRN-associated protein complexes. We found that a WRN variant containing a phenylalanine residue at position 1074 and an arginine at Oncotarget85680-85696https://www.ebi.ac.uk/biostudies/studies/S-EPMC5349866biostudies-literatureDifferent non-synonymous polymorphisms modulate the interaction of the WRN protein to its protein partners and its enzymatic activities.PMC5349866Garand CCaron MCPoirier GGTsofack SPCoulombe YLebel MMasson JYGagne JPLachapelle SfalseDifferent non-synonymous polymorphisms modulate the interaction of the WRN protein to its protein partners and its enzymatic activities.Werner syndrome (WS) is characterized by the premature onset of several age-associated pathologies including cancer. The protein defective in WS patients (WRN) is a helicase/exonuclease involved in DNA replication and repair. Here, we present the results of a large-scale proteome analysis that has been undertaken to determine protein partners of different polymorphic WRN proteins found with relatively high prevalence in the human population. We expressed different fluorescently tagged-WRN (eYFP-WRN) variants in human 293 embryonic kidney cells (HEK293) and used a combination of affinity-purification and mass spectrometry to identify different compositions of WRN-associated protein complexes. We found that a WRN variant containing a phenylalanine residue at position 1074 and an arginine at 2016-01-01T00:00:00Z2016 Dec2026-05-05T18:22:56.215Z2019-03-27T02:38:37ZS-EPMC53498662786339910.18632/oncotarget.13341