biostudies-literatureUnknown10Guo W<h4>Background</h4>Peptidylarginine deiminase (PAD) catalyzes the conversion of arginine residues to citrulline residues, termed citrullination. Recent studies have suggested that PAD isoform 2 (PADI2) plays an important role in tumors, although its tumorigenic effect and mechanism are largely unknown.<h4>Materials and methods</h4>Immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were used to investigate the expression level of PADI2 in various tumor tissues and patient blood samples, respectively. MNK-45 and Bel-7402 tumor cell lines originating from gastric and liver tumors, respectively, were treated with anti-PADI2 siRNA, and the subsequent cell proliferation, apoptosis and migration were observed. Polymerase chain reaction (PCR) arrays, including Cancer PathwayFinder,OncoTargets and therapy1475-1485https://www.ebi.ac.uk/biostudies/studies/S-EPMC5352236biostudies-literatureInvestigating the expression, effect and tumorigenic pathway of PADI2 in tumors.PMC5352236Zheng YLi CXu BGuo WZhang XWang YMa FChang XfalseInvestigating the expression, effect and tumorigenic pathway of PADI2 in tumors.<h4>Background</h4>Peptidylarginine deiminase (PAD) catalyzes the conversion of arginine residues to citrulline residues, termed citrullination. Recent studies have suggested that PAD isoform 2 (PADI2) plays an important role in tumors, although its tumorigenic effect and mechanism are largely unknown.<h4>Materials and methods</h4>Immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were used to investigate the expression level of PADI2 in various tumor tissues and patient blood samples, respectively. MNK-45 and Bel-7402 tumor cell lines originating from gastric and liver tumors, respectively, were treated with anti-PADI2 siRNA, and the subsequent cell proliferation, apoptosis and migration were observed. Polymerase chain reaction (PCR) arrays, including Cancer PathwayFinder,2017-01-01T00:00:00Z20172025-04-26T17:32:21.891Z2019-03-27T02:38:43ZS-EPMC53522362833134110.2147/OTT.S92389