{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(2)"],"submitter":["Fan JB"],"pubmed_abstract":["AMP-activated protein kinase (AMPK) activation could protect osteoblasts from dexamethasone (Dex). This study aims to provoke AMPK activation via microRNA downregulation of its negative regulator protein kinase C ζ (PKCζ). Results show that microRNA-25-5p (miR-25-5p) targets PKCζ's 3' untranslated regions (UTRs). Forced-expression of miR-25 downregulated PKCζ and activated AMPK in human osteoblastic cells (OB-6 and hFOB1.19 lines), which thereafter protected cells from Dex. Reversely, expression of antagomiR-25, the miR-25 inhibitor, upregulated PKCζ and inhibited AMPK activation, exacerbating Dex damages. Notably, PKCζ shRNA knockdown similarly activated AMPK and protected osteoblastic cells from Dex. AMPK activation was required for miR-25-induced osteoblastic cell protection. AMPKα shRN"],"journal":["Oncotarget"],"pagination":["3226-3236"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5356877"],"repository":["biostudies-literature"],"pubmed_title":["microRNA-25 targets PKCζ and protects osteoblastic cells from dexamethasone via activating AMPK signaling."],"pmcid":["PMC5356877"],"pubmed_authors":["Yi H","Fan JB","Cui ZM","Zhu XH","Cui SY","Zhao JN","Liu W"],"additional_accession":[]},"is_claimable":false,"name":"microRNA-25 targets PKCζ and protects osteoblastic cells from dexamethasone via activating AMPK signaling.","description":"AMP-activated protein kinase (AMPK) activation could protect osteoblasts from dexamethasone (Dex). This study aims to provoke AMPK activation via microRNA downregulation of its negative regulator protein kinase C ζ (PKCζ). Results show that microRNA-25-5p (miR-25-5p) targets PKCζ's 3' untranslated regions (UTRs). Forced-expression of miR-25 downregulated PKCζ and activated AMPK in human osteoblastic cells (OB-6 and hFOB1.19 lines), which thereafter protected cells from Dex. Reversely, expression of antagomiR-25, the miR-25 inhibitor, upregulated PKCζ and inhibited AMPK activation, exacerbating Dex damages. Notably, PKCζ shRNA knockdown similarly activated AMPK and protected osteoblastic cells from Dex. AMPK activation was required for miR-25-induced osteoblastic cell protection. AMPKα shRN","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Jan","modification":"2025-04-04T14:33:29.189Z","creation":"2019-03-26T23:46:30Z"},"accession":"S-EPMC5356877","cross_references":{"pubmed":["27911275"],"doi":["10.18632/oncotarget.13698"]}}