{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yao SY"],"funding":["NINDS NIH HHS"],"pagination":["146-56"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5362965"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["229(1-2)"],"pubmed_abstract":["There are currently four known isoforms of nitric oxide synthase (NOS). Of these, neuronal NOS (nNOS) is known to be present exclusively in neurons, endothelial NOS (eNOS) in vascular endothelium, while the inducible form of NOS (iNOS) is known to be activated in oligodendrocytes, astrocytes and microglia. The fourth isoform, mitochondrial NOS (mtNOS), represents a post-translational modification of nNOS. Using western blotting and real time-PCR, we show induction and activation of nNOS following culture of oligodendrocyte progenitor cells (OPC) with lipopolysaccharide (LPS). Activation of nNOS results in accumulation of peroxynitrite and tyrosine nitration of proteins in oligodendrocytes resulting in reduced cell viability. Injection of LPS in vivo into the corpus callosum of rats leads t"],"journal":["Journal of neuroimmunology"],"pubmed_title":["In vitro and in vivo induction and activation of nNOS by LPS in oligodendrocytes."],"pmcid":["PMC5362965"],"funding_grant_id":["R21 NS058787","R21 NS047474"],"pubmed_authors":["Ljunggren-Rose A","Yao SY","Whetsell WO","Chandramohan N","Sriram S"],"additional_accession":[]},"is_claimable":false,"name":"In vitro and in vivo induction and activation of nNOS by LPS in oligodendrocytes.","description":"There are currently four known isoforms of nitric oxide synthase (NOS). Of these, neuronal NOS (nNOS) is known to be present exclusively in neurons, endothelial NOS (eNOS) in vascular endothelium, while the inducible form of NOS (iNOS) is known to be activated in oligodendrocytes, astrocytes and microglia. The fourth isoform, mitochondrial NOS (mtNOS), represents a post-translational modification of nNOS. Using western blotting and real time-PCR, we show induction and activation of nNOS following culture of oligodendrocyte progenitor cells (OPC) with lipopolysaccharide (LPS). Activation of nNOS results in accumulation of peroxynitrite and tyrosine nitration of proteins in oligodendrocytes resulting in reduced cell viability. Injection of LPS in vivo into the corpus callosum of rats leads t","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Dec","modification":"2025-06-01T03:24:49.14Z","creation":"2020-11-07T09:50:23Z"},"accession":"S-EPMC5362965","cross_references":{"pubmed":["20724006"],"doi":["10.1016/j.jneuroim.2010.07.023"]}}