biostudies-literaturePachon-Pena GMinisterio de Salud Carlos IIISpanish Ministry of Economy and Competitiveness-European Regional Development Fund“Miguel Servet” tenure track programFondo de Investigacio'n SanitariaSpanish Biomedical Research Center in Diabetes and Associated Metabolic DisordersNHLBI NIH HHSNational Institutes of Health National Heart Lung Blood InstituteERDF1080-1092https://www.ebi.ac.uk/biostudies/studies/S-EPMC5367967biostudies-literatureUnknown35(4)The clinical effectiveness of systemically administered human mesenchymal stem cells (hMSCs) depends on their capacity to engage vascular endothelium. hMSCs derived from bone marrow (BM-hMSCs) natively lack endothelial binding capacity, but express a CD44 glycovariant containing N-linked sialyllactosamines that can be α(1,3)-fucosylated using fucosyltransferase-VI (FTVI) to enforce sLe^X decorations, thereby creating hematopoietic cell E-/L-selectin ligand (HCELL). HCELL expression programs potent shear-resistant adhesion of circulating cells to endothelial beds expressing E-selectin. An alternative source of hMSCs is adipose tissue (A-hMSCs), and we assessed whether A-hMSCs bind E-selectin and/or possess sialyllactosamine-decorated CD44 accessible to α(1,3)-fucosylation. SimilarStem cells (Dayton, Ohio)A Glycovariant of Human CD44 is Characteristically Expressed on Human Mesenchymal Stem Cells.PMC5367967CB07708/0012P01 HL107146PI14/00228PI11/0085CP10/00438CD10/00285SAF2012-36186NHLBI grant PO1 HL107146SAF2015-65019-RPachon-Pena GVendrell JKatz AFernandez-Veledo SSackstein RDonnelly CRuiz-Canada CfalseA Glycovariant of Human CD44 is Characteristically Expressed on Human Mesenchymal Stem Cells.The clinical effectiveness of systemically administered human mesenchymal stem cells (hMSCs) depends on their capacity to engage vascular endothelium. hMSCs derived from bone marrow (BM-hMSCs) natively lack endothelial binding capacity, but express a CD44 glycovariant containing N-linked sialyllactosamines that can be α(1,3)-fucosylated using fucosyltransferase-VI (FTVI) to enforce sLe^X decorations, thereby creating hematopoietic cell E-/L-selectin ligand (HCELL). HCELL expression programs potent shear-resistant adhesion of circulating cells to endothelial beds expressing E-selectin. An alternative source of hMSCs is adipose tissue (A-hMSCs), and we assessed whether A-hMSCs bind E-selectin and/or possess sialyllactosamine-decorated CD44 accessible to α(1,3)-fucosylation. Similar2017-01-01T00:00:00Z2017 Apr2025-04-04T02:12:07.773Z2019-03-26T23:22:10ZS-EPMC53679672788860210.1002/stem.2549