biostudies-literatureSim BWKRIBB Research Initiative Program34https://www.ebi.ac.uk/biostudies/studies/S-EPMC5368936biostudies-literatureUnknown17(1)<h4>Background</h4>Placental defects in somatic cell nuclear transfer (SCNT) are a major cause of complications during pregnancy. One of the most critical factors for the success of SCNT is the successful epigenetic reprogramming of donor cells. Recently, it was reported that the placental weight in mice cloned with the aggregated SCNT method was significantly reduced. Here, we examine the profile of abnormal gene expression using microarray technology in both regular SCNT and aggregated SCNT placentas as well as in vivo fertilization placentas. One SCNT embryo was aggregated with two 2 to 4 -cell stage tetraploid embryos from B6D2F1 mice (C57BL/6 × DBA/2).<h4>Results</h4>In SCNT placentas, 206 (1.6%) of the 12,816 genes probed were either up-regulated or down-regulated by more than two-foBMC biotechnologyAbnormal gene expression in regular and aggregated somatic cell nuclear transfer placentas.PMC5368936KGM4251622Sim BWMin KSPark CWKang MHfalseAbnormal gene expression in regular and aggregated somatic cell nuclear transfer placentas.<h4>Background</h4>Placental defects in somatic cell nuclear transfer (SCNT) are a major cause of complications during pregnancy. One of the most critical factors for the success of SCNT is the successful epigenetic reprogramming of donor cells. Recently, it was reported that the placental weight in mice cloned with the aggregated SCNT method was significantly reduced. Here, we examine the profile of abnormal gene expression using microarray technology in both regular SCNT and aggregated SCNT placentas as well as in vivo fertilization placentas. One SCNT embryo was aggregated with two 2 to 4 -cell stage tetraploid embryos from B6D2F1 mice (C57BL/6 × DBA/2).<h4>Results</h4>In SCNT placentas, 206 (1.6%) of the 12,816 genes probed were either up-regulated or down-regulated by more than two-fo2017-01-01T00:00:00Z2017 Mar2026-04-08T16:25:49.641Z2019-03-27T02:39:43ZS-EPMC53689362834730510.1186/s12896-017-0355-4