biostudies-literatureRueda-Lopez MMinisterio de Economía y CompetitividadConsejería de Economía, Innovación, Ciencia y Empleo, Junta de Andalucíae0174748https://www.ebi.ac.uk/biostudies/studies/S-EPMC5380328biostudies-literatureUnknown12(4)In this work, the role of the pine transcriptional regulator Dof 5 in carbon and nitrogen metabolism has been examined in poplar trees. The overexpression of the gene and potential effects on growth and biomass production were compared between trees growing in a growth chamber under controlled conditions and trees growing in a field trial during two growth seasons. Ten-week-old transgenic poplars exhibited higher growth than untransformed controls and exhibited enhanced capacity for inorganic nitrogen uptake in the form of nitrate. Furthermore, the transgenic trees accumulated significantly more carbohydrates such as glucose, fructose, sucrose and starch. Lignin content increased in the basal part of the stem likely due to the thicker stem of the transformed plants. The enhanced levels of PloS oneOverexpression of a pine Dof transcription factor in hybrid poplars: A comparative study in trees growing under controlled and natural conditions.PMC5380328BIO-474BIO2015-69285-RRueda-Lopez MPascual MBJauregui IAvila CLasa BHenao LMPallero MAparicio-Tejo PMCanovas FMfalseOverexpression of a pine Dof transcription factor in hybrid poplars: A comparative study in trees growing under controlled and natural conditions.In this work, the role of the pine transcriptional regulator Dof 5 in carbon and nitrogen metabolism has been examined in poplar trees. The overexpression of the gene and potential effects on growth and biomass production were compared between trees growing in a growth chamber under controlled conditions and trees growing in a field trial during two growth seasons. Ten-week-old transgenic poplars exhibited higher growth than untransformed controls and exhibited enhanced capacity for inorganic nitrogen uptake in the form of nitrate. Furthermore, the transgenic trees accumulated significantly more carbohydrates such as glucose, fructose, sucrose and starch. Lignin content increased in the basal part of the stem likely due to the thicker stem of the transformed plants. The enhanced levels of 2017-01-01T00:00:00Z20172026-07-16T05:22:29.373Z2019-03-26T22:53:02ZS-EPMC53803282837610010.1371/journal.pone.0174748