{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ding PH"],"funding":["The Hong Kong Research Grants Council","The University of Hong Kong (HKU)"],"pagination":["e0173223"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5383028"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(4)"],"pubmed_abstract":["Lipopolysaccharide (LPS)-binding protein (LBP) as an acute-phase protein plays a crucial role in innate host response to bacterial challenge. Our previous study shows that LBP expression in human gingiva is associated with periodontal status. Porphyromonas gingivalis is a keystone periodontopathogen, and its LPS with lipid A structural heterogeneity critically accounts for periodontal pathogenesis. This study investigated the effects of LBP and its interactions with two featured isoforms of P. gingivalis LPS (tetra-acylated LPS1435/1449 and penta-acylated LPS1690) on the expression of pro-inflammatory cytokines in human oral keratinocytes (HOKs), and the involvement of Toll-like receptor (TLR) signaling. HOKs were pre-incubated with recombinant human LBP (rhLBP) at 10ng/ml, 100ng/ml and 1μ"],"journal":["PloS one"],"pubmed_title":["3LPS-binding protein and its interactions with P. gingivalis LPS modulate pro-inflammatory response and Toll-like receptor signaling in human oral keratinocytes."],"pmcid":["PMC5383028"],"funding_grant_id":["GRF 768411M, 768713M and 17155216","The Modern Dental Laboratory/HKU Endowment Fund"],"pubmed_authors":["Wang CY","Ding PH","Darveau RP","Jin L"],"additional_accession":[]},"is_claimable":false,"name":"3LPS-binding protein and its interactions with P. gingivalis LPS modulate pro-inflammatory response and Toll-like receptor signaling in human oral keratinocytes.","description":"Lipopolysaccharide (LPS)-binding protein (LBP) as an acute-phase protein plays a crucial role in innate host response to bacterial challenge. Our previous study shows that LBP expression in human gingiva is associated with periodontal status. Porphyromonas gingivalis is a keystone periodontopathogen, and its LPS with lipid A structural heterogeneity critically accounts for periodontal pathogenesis. This study investigated the effects of LBP and its interactions with two featured isoforms of P. gingivalis LPS (tetra-acylated LPS1435/1449 and penta-acylated LPS1690) on the expression of pro-inflammatory cytokines in human oral keratinocytes (HOKs), and the involvement of Toll-like receptor (TLR) signaling. HOKs were pre-incubated with recombinant human LBP (rhLBP) at 10ng/ml, 100ng/ml and 1μ","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2025-04-26T11:11:04.239Z","creation":"2019-06-06T17:17:11Z"},"accession":"S-EPMC5383028","cross_references":{"pubmed":["28384159"],"doi":["10.1371/journal.pone.0173223"]}}