{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kulak NA"],"funding":["Novo Nordisk UK Research Foundation","Novo Nordisk Foundation Center for Protein Research","Novo Nordisk Fonden","Max-Planck-Gesellschaft"],"pagination":["694-705"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5383787"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(4)"],"pubmed_abstract":["Recent advances in mass spectrometry (MS)-based proteomics now allow very deep coverage of cellular proteomes. To achieve near-comprehensive identification and quantification, the combination of a first HPLC-based peptide fractionation orthogonal to the on-line LC-MS/MS step has proven to be particularly powerful. This first dimension is typically performed with milliliter/min flow and relatively large column inner diameters, which allow efficient pre-fractionation but typically require peptide amounts in the milligram range. Here, we describe a novel approach termed \"spider fractionator\" in which the post-column flow of a nanobore chromatography system enters an eight-port flow-selector rotor valve. The valve switches the flow into different flow channels at constant time intervals, such "],"journal":["Molecular & cellular proteomics : MCP"],"pubmed_title":["Loss-less Nano-fractionator for High Sensitivity, High Coverage Proteomics."],"pmcid":["PMC5383787"],"funding_grant_id":["NNF15SA0018240","PI Matthias Mann","NNF15CC0001"],"pubmed_authors":["Kulak NA","Geyer PE","Mann M"],"additional_accession":[]},"is_claimable":false,"name":"Loss-less Nano-fractionator for High Sensitivity, High Coverage Proteomics.","description":"Recent advances in mass spectrometry (MS)-based proteomics now allow very deep coverage of cellular proteomes. To achieve near-comprehensive identification and quantification, the combination of a first HPLC-based peptide fractionation orthogonal to the on-line LC-MS/MS step has proven to be particularly powerful. This first dimension is typically performed with milliliter/min flow and relatively large column inner diameters, which allow efficient pre-fractionation but typically require peptide amounts in the milligram range. Here, we describe a novel approach termed \"spider fractionator\" in which the post-column flow of a nanobore chromatography system enters an eight-port flow-selector rotor valve. The valve switches the flow into different flow channels at constant time intervals, such ","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Apr","modification":"2026-05-30T01:28:42.429Z","creation":"2019-03-27T02:40:40Z"},"accession":"S-EPMC5383787","cross_references":{"pubmed":["28126900"],"doi":["10.1074/mcp.O116.065136"]}}