{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Huang G"],"funding":["NIAAA NIH HHS"],"pagination":["7344"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5384283"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["4"],"pubmed_abstract":["A sensitive DNA isothermal amplification method for the detection of DNA at fM to aM concentrations for pathogen identification was developed using a non-stick-coated metal microfluidic bioreactor. A portable confocal optical detector was utilized to monitor the DNA amplification in micro- to nanoliter reaction assays in real-time, with fluorescence collection near the optical diffraction limit. The non-stick-coated metal microfluidic bioreactor, with a surface contact angle of 103°, was largely inert to bio-molecules, and DNA amplification could be performed in a minimum reaction volume of 40 nL. The isothermal nucleic acid amplification for Mycoplasma pneumoniae identification in the non-stick-coated microfluidic bioreactor could be performed at a minimum DNA template concentration of 1."],"journal":["Scientific reports"],"pubmed_title":["fM to aM nucleic acid amplification for molecular diagnostics in a non-stick-coated metal microfluidic bioreactor."],"pmcid":["PMC5384283"],"funding_grant_id":["K01 AA020102"],"pubmed_authors":["Fu R","Pang B","Ma L","Zhang Z","Wang R","Zhang J","Huang G","Li Q","Luo X","Huang Q","Ye J"],"additional_accession":[]},"is_claimable":false,"name":"fM to aM nucleic acid amplification for molecular diagnostics in a non-stick-coated metal microfluidic bioreactor.","description":"A sensitive DNA isothermal amplification method for the detection of DNA at fM to aM concentrations for pathogen identification was developed using a non-stick-coated metal microfluidic bioreactor. A portable confocal optical detector was utilized to monitor the DNA amplification in micro- to nanoliter reaction assays in real-time, with fluorescence collection near the optical diffraction limit. The non-stick-coated metal microfluidic bioreactor, with a surface contact angle of 103°, was largely inert to bio-molecules, and DNA amplification could be performed in a minimum reaction volume of 40 nL. The isothermal nucleic acid amplification for Mycoplasma pneumoniae identification in the non-stick-coated microfluidic bioreactor could be performed at a minimum DNA template concentration of 1.","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Dec","modification":"2026-05-30T01:34:36.731Z","creation":"2019-03-27T02:40:43Z"},"accession":"S-EPMC5384283","cross_references":{"pubmed":["25475544"],"doi":["10.1038/srep07344"]}}