biostudies-literatureHuang GNIAAA NIH HHS7344https://www.ebi.ac.uk/biostudies/studies/S-EPMC5384283biostudies-literatureUnknown4A sensitive DNA isothermal amplification method for the detection of DNA at fM to aM concentrations for pathogen identification was developed using a non-stick-coated metal microfluidic bioreactor. A portable confocal optical detector was utilized to monitor the DNA amplification in micro- to nanoliter reaction assays in real-time, with fluorescence collection near the optical diffraction limit. The non-stick-coated metal microfluidic bioreactor, with a surface contact angle of 103°, was largely inert to bio-molecules, and DNA amplification could be performed in a minimum reaction volume of 40 nL. The isothermal nucleic acid amplification for Mycoplasma pneumoniae identification in the non-stick-coated microfluidic bioreactor could be performed at a minimum DNA template concentration of 1.Scientific reportsfM to aM nucleic acid amplification for molecular diagnostics in a non-stick-coated metal microfluidic bioreactor.PMC5384283K01 AA020102Fu RPang BMa LZhang ZWang RZhang JHuang GLi QLuo XHuang QYe JfalsefM to aM nucleic acid amplification for molecular diagnostics in a non-stick-coated metal microfluidic bioreactor.A sensitive DNA isothermal amplification method for the detection of DNA at fM to aM concentrations for pathogen identification was developed using a non-stick-coated metal microfluidic bioreactor. A portable confocal optical detector was utilized to monitor the DNA amplification in micro- to nanoliter reaction assays in real-time, with fluorescence collection near the optical diffraction limit. The non-stick-coated metal microfluidic bioreactor, with a surface contact angle of 103°, was largely inert to bio-molecules, and DNA amplification could be performed in a minimum reaction volume of 40 nL. The isothermal nucleic acid amplification for Mycoplasma pneumoniae identification in the non-stick-coated microfluidic bioreactor could be performed at a minimum DNA template concentration of 1.2014-01-01T00:00:00Z2014 Dec2026-05-30T01:34:36.731Z2019-03-27T02:40:43ZS-EPMC53842832547554410.1038/srep07344