biostudies-literaturePhillips-Jones MKBiotechnology and Biological Sciences Research Council46180https://www.ebi.ac.uk/biostudies/studies/S-EPMC5387412biostudies-literatureUnknown7VanA-type resistance to glycopeptide antibiotics in clinical enterococci is regulated by the VanS_AR_A two-component signal transduction system. The nature of the molecular ligand that is recognised by the VanS_A sensory component has not hitherto been identified. Here we employ purified, intact and active VanS_A membrane protein (henceforth referred to as VanS) in analytical ultracentrifugation experiments to study VanS oligomeric state and conformation in the absence and presence of vancomycin. A combination of sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge (SEDFIT, SEDFIT-MSTAR and MULTISIG analysis) showed that VanS in the absence of the ligand is almost entirely monomeric (molar mass M = 45.7 kDa) in dilutScientific reportsHydrodynamics of the VanA-type VanS histidine kinase: an extended solution conformation and first evidence for interactions with vancomycin.PMC5387412BB/M013081/1BB/D001641/1Hughes CSDinu VAdams GGPhillips-Jones MKGillis RBChannell GKelsall CJPatching SGHarding SEAshcroft AEfalseHydrodynamics of the VanA-type VanS histidine kinase: an extended solution conformation and first evidence for interactions with vancomycin.VanA-type resistance to glycopeptide antibiotics in clinical enterococci is regulated by the VanS_AR_A two-component signal transduction system. The nature of the molecular ligand that is recognised by the VanS_A sensory component has not hitherto been identified. Here we employ purified, intact and active VanS_A membrane protein (henceforth referred to as VanS) in analytical ultracentrifugation experiments to study VanS oligomeric state and conformation in the absence and presence of vancomycin. A combination of sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge (SEDFIT, SEDFIT-MSTAR and MULTISIG analysis) showed that VanS in the absence of the ligand is almost entirely monomeric (molar mass M = 45.7 kDa) in dilut2017-01-01T00:00:00Z2017 Apr2025-04-26T04:49:33.954Z2019-03-27T02:41:01ZS-EPMC53874122839785310.1038/srep46180