{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lupu DS"],"funding":["National Institute of Diabetes and Digestive and Kidney Diseases","NIDDK NIH HHS","NIEHS NIH HHS","National Institutes of Health"],"pagination":["2090-2103"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5388550"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["31(5)"],"pubmed_abstract":["Folate B<sub>12</sub>-dependent remethylation of homocysteine is important, but less is understood about the importance of the alternative betaine-dependent methylation pathway-catalyzed by betaine-homocysteine methyltransferase (BHMT)-for establishing and maintaining adequate DNA methylation across the genome. We studied C57Bl/6J <i>Bhmt</i> (betaine-homocysteine methyltransferase)-null mice at age 4, 12, 24, and 52 wk (<i>N</i> = 8) and observed elevation of <i>S</i>-adenosylhomocysteine concentrations and development of preneoplastic foci in the liver (increased placental glutathione <i>S</i>-transferase and cytokeratin 8-18 activity; starting at 12 wk). At 4 wk, we identified 63 differentially methylated CpGs (DMCs; false discovery rate < 5%) proximal to 81 genes (across 14 chromosomes"],"journal":["FASEB journal : official publication of the Federation of American Societies for Experimental Biology"],"pubmed_title":["Altered methylation of specific DNA loci in the liver of <i>Bhmt</i>-null mice results in repression of <i>Iqgap2</i> and <i>F2rl2</i> and is associated with development of preneoplastic foci."],"pmcid":["PMC5388550"],"funding_grant_id":["DK56350","P30 DK056350","P30 ES010126"],"pubmed_authors":["Lupu DS","Pellegrini M","Orozco LD","Cullen JM","Zeisel SH","Wang Y"],"additional_accession":[]},"is_claimable":false,"name":"Altered methylation of specific DNA loci in the liver of <i>Bhmt</i>-null mice results in repression of <i>Iqgap2</i> and <i>F2rl2</i> and is associated with development of preneoplastic foci.","description":"Folate B<sub>12</sub>-dependent remethylation of homocysteine is important, but less is understood about the importance of the alternative betaine-dependent methylation pathway-catalyzed by betaine-homocysteine methyltransferase (BHMT)-for establishing and maintaining adequate DNA methylation across the genome. We studied C57Bl/6J <i>Bhmt</i> (betaine-homocysteine methyltransferase)-null mice at age 4, 12, 24, and 52 wk (<i>N</i> = 8) and observed elevation of <i>S</i>-adenosylhomocysteine concentrations and development of preneoplastic foci in the liver (increased placental glutathione <i>S</i>-transferase and cytokeratin 8-18 activity; starting at 12 wk). At 4 wk, we identified 63 differentially methylated CpGs (DMCs; false discovery rate < 5%) proximal to 81 genes (across 14 chromosomes","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 May","modification":"2026-05-30T07:18:35.422Z","creation":"2019-03-26T23:33:50Z"},"accession":"S-EPMC5388550","cross_references":{"pubmed":["28179424"],"doi":["10.1096/fj.201601169R","10.1096/fj.201601169r"]}}