biostudies-literatureUnknown8(1-2)Yang YOsteosarcoma is the most common primary malignant bone tumor in children and young adults. Although histologically defined by the presence of malignant osteoid, the tumor possesses lineage multipotency suggesting it could be derived from a cell anywhere on the differentiation pathway between a mesenchymal stem cell (MSC) and a mature osteoblast. To determine if preosteoblasts (pOB) could be the cell of origin differentiated MSCs were transformed with defined genetic elements. MSCs and pOB differentiated from the same MSCs were serially transformed with the oncogenes hTERT, SV40 large T antigen and H-Ras. Assays were performed to determine their tumorigenic properties, differentiation capacity and histologic appearance. When subcutaneously implanted in immunocompromised mice, cell lines derGenes & cancer484-494https://www.ebi.ac.uk/biostudies/studies/S-EPMC5396624biostudies-literatureGenetically transforming human osteoblasts to sarcoma: development of an osteosarcoma model.PMC5396624Hoang BRoth MRao PGorlick RFreeman CTripathi SGill JZhang WDorfman HYang YRosenblum JZhang YSowers RYang RPiperdi SGeller DPark AfalseGenetically transforming human osteoblasts to sarcoma: development of an osteosarcoma model.Osteosarcoma is the most common primary malignant bone tumor in children and young adults. Although histologically defined by the presence of malignant osteoid, the tumor possesses lineage multipotency suggesting it could be derived from a cell anywhere on the differentiation pathway between a mesenchymal stem cell (MSC) and a mature osteoblast. To determine if preosteoblasts (pOB) could be the cell of origin differentiated MSCs were transformed with defined genetic elements. MSCs and pOB differentiated from the same MSCs were serially transformed with the oncogenes hTERT, SV40 large T antigen and H-Ras. Assays were performed to determine their tumorigenic properties, differentiation capacity and histologic appearance. When subcutaneously implanted in immunocompromised mice, cell lines der2017-01-01T00:00:00Z2017 Jan2025-04-26T18:55:41.973Z2019-03-27T02:41:35ZS-EPMC53966242843552010.18632/genesandcancer.133