{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(4)"],"submitter":["Liu J"],"pubmed_abstract":["Cytokine-induced killer cells (CIK) have been used in clinic for adoptive immunotherapy in a variety of malignant tumors and have improved the prognosis of cancer patients. However, there are individual differences in the CIK cell preparations including the obvious differences in the ratio of effector CIK cells among different cancer patients. Infusion of such heterogeneous immune cell preparation is an important factor that would affect the therapeutic efficacy. We report here the enrichment and expansion of CD8+ cells from CIK cells cultured for one week using magnetic activated cell sorting (MACS). These enriched CD8+ CIK cells expressed T cell marker CD3 and antigen recognition receptor NKG2D. Phenotypic analysis showed that CD8+ CIK cells contained 32.4% of CD3+ CD56+ natural killer ("],"journal":["PloS one"],"pagination":["e0175704"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5398541"],"repository":["biostudies-literature"],"pubmed_title":["Phenotypic characterization and anticancer capacity of CD8+ cytokine-induced killer cells after antigen-induced expansion."],"pmcid":["PMC5398541"],"pubmed_authors":["Zhang W","Liu J","Cao Y","Wang Y","Wang L"],"additional_accession":[]},"is_claimable":false,"name":"Phenotypic characterization and anticancer capacity of CD8+ cytokine-induced killer cells after antigen-induced expansion.","description":"Cytokine-induced killer cells (CIK) have been used in clinic for adoptive immunotherapy in a variety of malignant tumors and have improved the prognosis of cancer patients. However, there are individual differences in the CIK cell preparations including the obvious differences in the ratio of effector CIK cells among different cancer patients. Infusion of such heterogeneous immune cell preparation is an important factor that would affect the therapeutic efficacy. We report here the enrichment and expansion of CD8+ cells from CIK cells cultured for one week using magnetic activated cell sorting (MACS). These enriched CD8+ CIK cells expressed T cell marker CD3 and antigen recognition receptor NKG2D. Phenotypic analysis showed that CD8+ CIK cells contained 32.4% of CD3+ CD56+ natural killer (","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2025-04-04T18:44:08.037Z","creation":"2019-03-26T22:49:41Z"},"accession":"S-EPMC5398541","cross_references":{"pubmed":["28426690"],"doi":["10.1371/journal.pone.0175704"]}}