biostudies-literatureUnknown46(2)Fallahzadeh-Abarghooei L<h4>Background</h4>Sodium arsenite (NaAsO2) has potent cytotoxic activity in human cancer cells. Oxidative stress has been suggested as a mechanism for arsenic-induced carcinogenesis. The purpose of the present study was to evaluate the alteration of mRNA levels of catalase (<i>CAT</i>) and superoxide dismutase 2 (<i>SOD2</i>) in MCF-7 and Jurkat cells after exposure to NaAsO₂.<h4>Methods</h4>Methylthiazol tetrazolium (MTT) viability assay was performed to evaluate cytotoxicity of NaAsO₂ in MCF-7 and Jurkat cells. For evaluating the expression levels of the <i>CAT</i> and <i>SOD2</i>, we used two concentrations of NaAsO₂ (5 and 15 μM), lower than the concentrations at which 50% of cell viability were lost. The cells were treated with co-treatment of NaAsO<sIranian journal of public health229-234https://www.ebi.ac.uk/biostudies/studies/S-EPMC5402782biostudies-literatureEffect of Sodium Arsenite on the Expression of Antioxidant Genes (<i>SOD2</i> and <i>CAT</i>) in MCF-7 and Jurkat Cell Lines.PMC5402782Fallahzadeh-Abarghooei LSaadat MSamadaei-Ghadikolaie MSaadat IfalseEffect of Sodium Arsenite on the Expression of Antioxidant Genes (<i>SOD2</i> and <i>CAT</i>) in MCF-7 and Jurkat Cell Lines.<h4>Background</h4>Sodium arsenite (NaAsO2) has potent cytotoxic activity in human cancer cells. Oxidative stress has been suggested as a mechanism for arsenic-induced carcinogenesis. The purpose of the present study was to evaluate the alteration of mRNA levels of catalase (<i>CAT</i>) and superoxide dismutase 2 (<i>SOD2</i>) in MCF-7 and Jurkat cells after exposure to NaAsO₂.<h4>Methods</h4>Methylthiazol tetrazolium (MTT) viability assay was performed to evaluate cytotoxicity of NaAsO₂ in MCF-7 and Jurkat cells. For evaluating the expression levels of the <i>CAT</i> and <i>SOD2</i>, we used two concentrations of NaAsO₂ (5 and 15 μM), lower than the concentrations at which 50% of cell viability were lost. The cells were treated with co-treatment of NaAsO<s2017-01-01T00:00:00Z2017 Feb2025-04-05T12:54:45.371Z2019-06-06T17:21:57ZS-EPMC540278228451559