biostudies-literatureYang GNIBIB NIH HHSU.S. Department of DefensePennsylvania Department of HealthNational Institutes of HealthNIAMS NIH HHSNational Institute of Biomedical Imaging and Bioengineering68-76https://www.ebi.ac.uk/biostudies/studies/S-EPMC5408748biostudies-literatureUnknown35Regeneration of injured tendon and ligament (T&L) remains a clinical challenge due to their poor intrinsic healing capacity. Tissue engineering provides a promising alternative treatment approach to facilitate T&L healing and regeneration. Successful tendon tissue engineering requires the use of three-dimensional (3D) biomimetic scaffolds that possess the physical and biochemical features of native tendon tissue. We report here the development and characterization of a novel composite scaffold fabricated by co-electrospinning of poly-ε-caprolactone (PCL) and methacrylated gelatin (mGLT). We found that photocrosslinking retained mGLT, resulted in a uniform distribution of mGLT throughout the depth of scaffold and also preserved scaffold mechanical strength. Moreover, photocrosslinking was aActa biomaterialiaMultilayered polycaprolactone/gelatin fiber-hydrogel composite for tendon tissue engineering.PMC5408748W81XWH-08-2-0032W81XWH-15-1-0104SAP 4100050913R01 AR062947T32EB0010265R01 AR062947W81XWH-11-2-0143T32 EB001026W81XWH-14-2-0003Yang GRothrauff BBYu STuan RSLin HfalseMultilayered polycaprolactone/gelatin fiber-hydrogel composite for tendon tissue engineering.Regeneration of injured tendon and ligament (T&L) remains a clinical challenge due to their poor intrinsic healing capacity. Tissue engineering provides a promising alternative treatment approach to facilitate T&L healing and regeneration. Successful tendon tissue engineering requires the use of three-dimensional (3D) biomimetic scaffolds that possess the physical and biochemical features of native tendon tissue. We report here the development and characterization of a novel composite scaffold fabricated by co-electrospinning of poly-ε-caprolactone (PCL) and methacrylated gelatin (mGLT). We found that photocrosslinking retained mGLT, resulted in a uniform distribution of mGLT throughout the depth of scaffold and also preserved scaffold mechanical strength. Moreover, photocrosslinking was a2016-01-01T00:00:00Z2016 Apr2025-04-04T13:14:30.497Z2019-03-27T02:42:15ZS-EPMC54087482694563110.1016/j.actbio.2016.03.004