{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Park GW"],"pubmed_abstract":["Human noroviruses are a leading cause of epidemic and sporadic gastroenteritis worldwide. Because most infections are either spread directly via the person-to-person route or indirectly through environmental surfaces or food, contaminated fomites and inanimate surfaces are important vehicles for the spread of the virus during norovirus outbreaks. We developed and evaluated a protocol using macrofoam swabs for the detection and typing of human noroviruses from hard surfaces. Compared with fiber-tipped swabs or antistatic wipes, macrofoam swabs allow virus recovery (range 1.2-33.6%) from toilet seat surfaces of up to 700 cm<sup>2</sup>. The protocol includes steps for the extraction of the virus from the swabs and further concentration of the viral RNA using spin columns. In total, 127 (58.5"],"journal":["Journal of visualized experiments : JoVE"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5409301"],"repository":["biostudies-literature"],"pubmed_title":["Swab Sampling Method for the Detection of Human Norovirus on Surfaces."],"pmcid":["PMC5409301"],"pubmed_authors":["Vinje J","Park GW","Chhabra P"],"additional_accession":[]},"is_claimable":false,"name":"Swab Sampling Method for the Detection of Human Norovirus on Surfaces.","description":"Human noroviruses are a leading cause of epidemic and sporadic gastroenteritis worldwide. Because most infections are either spread directly via the person-to-person route or indirectly through environmental surfaces or food, contaminated fomites and inanimate surfaces are important vehicles for the spread of the virus during norovirus outbreaks. We developed and evaluated a protocol using macrofoam swabs for the detection and typing of human noroviruses from hard surfaces. Compared with fiber-tipped swabs or antistatic wipes, macrofoam swabs allow virus recovery (range 1.2-33.6%) from toilet seat surfaces of up to 700 cm<sup>2</sup>. The protocol includes steps for the extraction of the virus from the swabs and further concentration of the viral RNA using spin columns. In total, 127 (58.5","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Feb","modification":"2026-07-07T03:11:18.959Z","creation":"2026-07-07T03:07:29.909Z"},"accession":"S-EPMC5409301","cross_references":{"pubmed":["28287539"],"doi":["10.3791/55205"]}}