{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6"],"submitter":["Zhang X"],"pubmed_abstract":["Recent research shows surging interest to visualize human G protein-coupled receptor (GPCR) dynamic structures using the bottom-up H/D-exchange (HDX) proteomics technology. This opinion article clarifies critical technical nuances and logical thinking behind the GPCR HDX proteomics method, to help scientists overcome cross-discipline pitfalls, and understand and reproduce the protocol at high quality. The 2010 89% HDX structural coverage of GPCR was achieved with both structural and analytical rigor. This article emphasizes systematically considering membrane protein structure stability and compatibility with chromatography and mass spectrometry (MS) throughout the pipeline, including the effects of metal ions, zero-detergent shock, and freeze-thaws on HDX result rigor. This article propos"],"journal":["F1000Research"],"pagination":["89"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5428523"],"repository":["biostudies-literature"],"pubmed_title":["Seven perspectives on GPCR H/D-exchange proteomics methods."],"pmcid":["PMC5428523"],"pubmed_authors":["Zhang X"],"additional_accession":[]},"is_claimable":false,"name":"Seven perspectives on GPCR H/D-exchange proteomics methods.","description":"Recent research shows surging interest to visualize human G protein-coupled receptor (GPCR) dynamic structures using the bottom-up H/D-exchange (HDX) proteomics technology. This opinion article clarifies critical technical nuances and logical thinking behind the GPCR HDX proteomics method, to help scientists overcome cross-discipline pitfalls, and understand and reproduce the protocol at high quality. The 2010 89% HDX structural coverage of GPCR was achieved with both structural and analytical rigor. This article emphasizes systematically considering membrane protein structure stability and compatibility with chromatography and mass spectrometry (MS) throughout the pipeline, including the effects of metal ions, zero-detergent shock, and freeze-thaws on HDX result rigor. This article propos","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2026-04-07T20:21:44.388Z","creation":"2019-03-27T02:43:22Z"},"accession":"S-EPMC5428523","cross_references":{"pubmed":["28529698"],"doi":["10.12688/f1000research.10667.1"]}}