{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(1)"],"submitter":["Muller MM"],"pubmed_abstract":["Exposure of human monocytes to lipopolysaccharide (LPS) induces a temporary insensitivity to subsequent LPS challenges, a cellular state called endotoxin tolerance. In this study, we investigated the LPS-induced global glycoprotein expression changes of tolerant human monocytes and THP-1 cells to identify markers and glycoprotein targets capable to modulate the immunosuppressive state. Using hydrazide chemistry and LC-MS/MS analysis, we analyzed glycoprotein expression changes during a 48 h LPS time course. The cellular snapshots at different time points identified 1491 glycoproteins expressed by monocytes and THP-1 cells. Label-free quantitative analysis revealed transient or long-lasting LPS-induced expression changes of secreted or membrane-anchored glycoproteins derived from intracellu"],"journal":["Scientific reports"],"pagination":["838"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5429802"],"repository":["biostudies-literature"],"pubmed_title":["Global analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and GPR84 as a modulator of TNFα expression."],"pmcid":["PMC5429802"],"pubmed_authors":["Behnert A","Guthke R","Muller MM","Slevogt H","Moore C","Klassert TE","Reifenstein S","Conrad T","Kuhn A","Driesch D","Lehmann R"],"additional_accession":[]},"is_claimable":false,"name":"Global analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and GPR84 as a modulator of TNFα expression.","description":"Exposure of human monocytes to lipopolysaccharide (LPS) induces a temporary insensitivity to subsequent LPS challenges, a cellular state called endotoxin tolerance. In this study, we investigated the LPS-induced global glycoprotein expression changes of tolerant human monocytes and THP-1 cells to identify markers and glycoprotein targets capable to modulate the immunosuppressive state. Using hydrazide chemistry and LC-MS/MS analysis, we analyzed glycoprotein expression changes during a 48 h LPS time course. The cellular snapshots at different time points identified 1491 glycoproteins expressed by monocytes and THP-1 cells. Label-free quantitative analysis revealed transient or long-lasting LPS-induced expression changes of secreted or membrane-anchored glycoproteins derived from intracellu","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Apr","modification":"2026-04-15T23:13:29.946Z","creation":"2019-03-27T02:43:28Z"},"accession":"S-EPMC5429802","cross_references":{"pubmed":["28404994"],"doi":["10.1038/s41598-017-00828-y"]}}