biostudies-literatureUnknown54(1)Li ZN<h4>Background</h4>Phytoplasmas are always associated with symptoms in host plants such as stunting of stems, witches'-broom, yellowing of leaves, formation of sterile-deformed flowers, virescence and phyllody. Recently also symptom of fasciation was reported associated with phytoplasma presence. In the present work, China ixeris fasciation was observed associated with phytoplasmas in Guanzhong Area, Shaanxi, China.<h4>Results</h4>Phytoplasma-like bodies were observed under transmission electron microscope in stem tissues of symptomatic samples. The 16S rRNA operon and tuf genes from phytoplasmas were amplified by PCR assays. Phylogenetic trees were calculated respectively based on sequences data of these two genes. The pattern of restriction fragment length polymorphism (RFLP) was generatBotanical studies52https://www.ebi.ac.uk/biostudies/studies/S-EPMC5430337biostudies-literatureDetection and identification of the phytoplasma associated with China ixeris (Ixeridium chinense) fasciation.PMC5430337Wu YFLi ZNLiu PZhang LfalseDetection and identification of the phytoplasma associated with China ixeris (Ixeridium chinense) fasciation.<h4>Background</h4>Phytoplasmas are always associated with symptoms in host plants such as stunting of stems, witches'-broom, yellowing of leaves, formation of sterile-deformed flowers, virescence and phyllody. Recently also symptom of fasciation was reported associated with phytoplasma presence. In the present work, China ixeris fasciation was observed associated with phytoplasmas in Guanzhong Area, Shaanxi, China.<h4>Results</h4>Phytoplasma-like bodies were observed under transmission electron microscope in stem tissues of symptomatic samples. The 16S rRNA operon and tuf genes from phytoplasmas were amplified by PCR assays. Phylogenetic trees were calculated respectively based on sequences data of these two genes. The pattern of restriction fragment length polymorphism (RFLP) was generat2013-01-01T00:00:00Z2013 Dec2026-07-07T03:12:36.942Z2019-06-06T17:27:32ZS-EPMC54303372851089710.1186/1999-3110-54-52