{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5"],"submitter":["Rashid RB"],"pubmed_abstract":["<i>Vibrio cholerae</i> O1 and O139 has been known for its ability to cause epidemics. These strains produce cholera toxin which is the main cause of secretory diarrhea. <i>V. cholerae</i> non-O1 and non-O139 strains are also capable of causing gastroenteritis as well as septicemia and peritonitis. It has been proven that virulence factors such as T6SS, <i>hapA, rtxA</i>, and <i>hlyA</i> are present in almost all <i>V. cholerae</i> strains. It is imperative that viable but non-culturable cells of <i>V. cholerae</i> are also detected since they are also known to cause diarrhea. Thus, the aim of this study was to develop an assay that detects all <i>V. cholerae</i> regardless of their serotype, culturable state, and virulence genes present, by targeting the species specific conserved <i>ompW</i> sequence. The developed assay meets these goals with 100% specificity and is capable of detecting as low as 5.46 copy number of <i>V. cholerae</i>. Detection is rapid since neither lengthy incubation period nor electrophoresis is required. The assay had excellent repeatability (CV%: 0.24-1.32) and remarkable reproducibility (CV%: 1.08-3.7). Amplification efficiencies in the 89-100% range were observed. The assay is more economical than Taqman-based multiplex real-time PCR assays. Compared to other real-time assays, the <i>ompW</i> assay is specific and sensitive, has better repeatability and reproducibility, and is more economical."],"journal":["Frontiers in public health"],"pagination":["109"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5437123"],"repository":["biostudies-literature"],"pubmed_title":["Development and Validation of a Novel Real-time Assay for the Detection and Quantification of <i>Vibrio cholerae</i>."],"pmcid":["PMC5437123"],"pubmed_authors":["Jensen PKM","Rashid RB","Begum A","Tulsiani S","Ferdous J"],"additional_accession":[]},"is_claimable":false,"name":"Development and Validation of a Novel Real-time Assay for the Detection and Quantification of <i>Vibrio cholerae</i>.","description":"<i>Vibrio cholerae</i> O1 and O139 has been known for its ability to cause epidemics. These strains produce cholera toxin which is the main cause of secretory diarrhea. <i>V. cholerae</i> non-O1 and non-O139 strains are also capable of causing gastroenteritis as well as septicemia and peritonitis. It has been proven that virulence factors such as T6SS, <i>hapA, rtxA</i>, and <i>hlyA</i> are present in almost all <i>V. cholerae</i> strains. It is imperative that viable but non-culturable cells of <i>V. cholerae</i> are also detected since they are also known to cause diarrhea. Thus, the aim of this study was to develop an assay that detects all <i>V. cholerae</i> regardless of their serotype, culturable state, and virulence genes present, by targeting the species specific conserved <i>ompW</i> sequence. The developed assay meets these goals with 100% specificity and is capable of detecting as low as 5.46 copy number of <i>V. cholerae</i>. Detection is rapid since neither lengthy incubation period nor electrophoresis is required. The assay had excellent repeatability (CV%: 0.24-1.32) and remarkable reproducibility (CV%: 1.08-3.7). Amplification efficiencies in the 89-100% range were observed. The assay is more economical than Taqman-based multiplex real-time PCR assays. Compared to other real-time assays, the <i>ompW</i> assay is specific and sensitive, has better repeatability and reproducibility, and is more economical.","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2024-11-21T03:15:54.277Z","creation":"2020-11-19T16:51:53Z"},"accession":"S-EPMC5437123","cross_references":{"pubmed":["28580353"],"doi":["10.3389/fpubh.2017.00109"]}}