{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Means TK"],"funding":["NIDDK NIH HHS","NCI NIH HHS","NIAMS NIH HHS"],"pagination":["407-17"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC544604"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["115(2)"],"pubmed_abstract":["Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by pathogenic autoantibodies against nucleoproteins and DNA. Here we show that DNA-containing immune complexes (ICs) within lupus serum (SLE-ICs), but not protein-containing ICs from other autoimmune rheumatic diseases, stimulates plasmacytoid DCs (PDCs) to produce cytokines and chemokines via a cooperative interaction between Toll-like receptor 9 (TLR9) and FcgammaRIIa (CD32). SLE-ICs transiently colocalized to a subcellular compartment containing CD32 and TLR9, and CD32+, but not CD32-, PDCs internalized and responded to SLE-ICs. Our findings demonstrate a novel functional interaction between Fc receptors and TLRs, defining a pathway in which CD32 delivers SLE-ICs to intracellular lysosomes containing TLR9, inducin"],"journal":["The Journal of clinical investigation"],"pubmed_title":["Human lupus autoantibody-DNA complexes activate DCs through cooperation of CD32 and TLR9."],"pmcid":["PMC544604"],"funding_grant_id":["5T32AR07258","P01-DK50305","P01 DK050305","R01-CA69212","T32 AR007258","R01 CA069212"],"pubmed_authors":["Luster AD","Means TK","Golenbock DT","Hayashi F","Murali MR","Latz E"],"additional_accession":[]},"is_claimable":false,"name":"Human lupus autoantibody-DNA complexes activate DCs through cooperation of CD32 and TLR9.","description":"Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by pathogenic autoantibodies against nucleoproteins and DNA. Here we show that DNA-containing immune complexes (ICs) within lupus serum (SLE-ICs), but not protein-containing ICs from other autoimmune rheumatic diseases, stimulates plasmacytoid DCs (PDCs) to produce cytokines and chemokines via a cooperative interaction between Toll-like receptor 9 (TLR9) and FcgammaRIIa (CD32). SLE-ICs transiently colocalized to a subcellular compartment containing CD32 and TLR9, and CD32+, but not CD32-, PDCs internalized and responded to SLE-ICs. Our findings demonstrate a novel functional interaction between Fc receptors and TLRs, defining a pathway in which CD32 delivers SLE-ICs to intracellular lysosomes containing TLR9, inducin","dates":{"release":"2005-01-01T00:00:00Z","publication":"2005 Feb","modification":"2025-04-20T01:34:41.243Z","creation":"2019-03-27T01:08:30Z"},"accession":"S-EPMC544604","cross_references":{"pubmed":["15668740"],"doi":["10.1172/jci200523025","10.1172/JCI23025"]}}