biostudies-literatureMizuguchi THoward Hughes Medical InstituteHHS | NIH | National Cancer Institute5479-5484https://www.ebi.ac.uk/biostudies/studies/S-EPMC5448194biostudies-literatureUnknown114(21)The dynamic nature of genome organization impacts critical nuclear functions including the regulation of gene expression, replication, and DNA damage repair. Despite significant progress, the mechanisms responsible for reorganization of the genome in response to cellular stress, such as aberrant DNA replication, are poorly understood. Here, we show that fission yeast cells carrying a mutation in the DNA-binding protein Sap1 show defects in DNA replication progression and genome stability and display extensive changes in genome organization. Chromosomal regions such as subtelomeres that show defects in replication progression associate with the nuclear envelope in <i>sap1</i> mutant cells. Moreover, high-resolution, genome-wide chromosome conformation capture (Hi-C) analysis revealed prominProceedings of the National Academy of Sciences of the United States of AmericaShelterin components mediate genome reorganization in response to replication stress.PMC54481948341225FitzGerald PMatsuda EBelton JMDekker JTaneja NGrewal SISMizuguchi TfalseShelterin components mediate genome reorganization in response to replication stress.The dynamic nature of genome organization impacts critical nuclear functions including the regulation of gene expression, replication, and DNA damage repair. Despite significant progress, the mechanisms responsible for reorganization of the genome in response to cellular stress, such as aberrant DNA replication, are poorly understood. Here, we show that fission yeast cells carrying a mutation in the DNA-binding protein Sap1 show defects in DNA replication progression and genome stability and display extensive changes in genome organization. Chromosomal regions such as subtelomeres that show defects in replication progression associate with the nuclear envelope in <i>sap1</i> mutant cells. Moreover, high-resolution, genome-wide chromosome conformation capture (Hi-C) analysis revealed promin2017-01-01T00:00:00Z2017 May2026-04-07T18:38:10.328Z2019-03-27T02:46:13ZS-EPMC54481942849049810.1073/pnas.1705527114