{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Silipo M"],"funding":["Medical Research Council"],"pagination":["536-543"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5449083"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(5)"],"pubmed_abstract":["The human epidermal growth factor receptor 2 (HER2)/receptor tyrosine-protein kinasebB-2 (ERBB2) is overexpressed in 20-30% of breast tumors leading to faster growing and more aggressive tumors. Alternative splicing generates a functionally distinct HER2 variant called Herstatin, which is produced by the inclusion of intron 8. Herstatin acts as a tumor suppressor by effectively blocking HER2 activity and cell proliferation, while promoting apoptosis. In the present study we investigated HER2 pre-mRNA regulatory sequences and splicing factors which regulate the alternative splicing of Herstatin. A Herstatin minigene, comprising exon 8/intron 8/exon 9 of HER2 was generated and subsequent in vitro splicing assays revealed that RNA secondary structure and somatic mutations did not impact on in"],"journal":["RNA biology"],"pubmed_title":["How is Herstatin, a tumor suppressor splice variant of the oncogene HER2, regulated?"],"pmcid":["PMC5449083"],"funding_grant_id":["MC_PC_14101"],"pubmed_authors":["Silipo M","Lennard T","Tyson-Capper A","Gautrey H","Satam S"],"additional_accession":[]},"is_claimable":false,"name":"How is Herstatin, a tumor suppressor splice variant of the oncogene HER2, regulated?","description":"The human epidermal growth factor receptor 2 (HER2)/receptor tyrosine-protein kinasebB-2 (ERBB2) is overexpressed in 20-30% of breast tumors leading to faster growing and more aggressive tumors. Alternative splicing generates a functionally distinct HER2 variant called Herstatin, which is produced by the inclusion of intron 8. Herstatin acts as a tumor suppressor by effectively blocking HER2 activity and cell proliferation, while promoting apoptosis. In the present study we investigated HER2 pre-mRNA regulatory sequences and splicing factors which regulate the alternative splicing of Herstatin. A Herstatin minigene, comprising exon 8/intron 8/exon 9 of HER2 was generated and subsequent in vitro splicing assays revealed that RNA secondary structure and somatic mutations did not impact on in","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 May","modification":"2025-04-04T12:36:26.828Z","creation":"2019-03-27T02:46:15Z"},"accession":"S-EPMC5449083","cross_references":{"pubmed":["27935425"],"doi":["10.1080/15476286.2016.1267074"]}}