{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(1)"],"submitter":["Li S"],"pubmed_abstract":["Genotype Nisqually-1 is the first model woody plant with an available well-annotated genome. Nevertheless, a simple and rapid transformation of Nisqually-1 remains to be established. Here, we developed a novel shoot regeneration method for Nisqually-1 using leaf petiole and stem segment explants. Numerous shoots formed in the incision of explants within two weeks. The optimized shoot regeneration medium (SRM) contained 0.03 mg l<sup>-1</sup> 6-benzylaminopurine, 0.02 mg l<sup>-1</sup> indole-3-butyric acid and 0.0008 mg l<sup>-1</sup> thidiazuron. Based on this, Agrobacterium-mediated genetic transformation of stem explants was examined using the vector pBI<sub>121</sub> that contains the β-glucuronidase (GUS) as a reporter gene. Consequently, factors affecting transformation frequency of "],"journal":["Scientific reports"],"pagination":["2638"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5453977"],"repository":["biostudies-literature"],"pubmed_title":["Simple, rapid and efficient transformation of genotype Nisqually-1: a basic tool for the first sequenced model tree."],"pmcid":["PMC5453977"],"pubmed_authors":["Li S","Wang C","Zhen C","Xu W","Cheng Y"],"additional_accession":[]},"is_claimable":false,"name":"Simple, rapid and efficient transformation of genotype Nisqually-1: a basic tool for the first sequenced model tree.","description":"Genotype Nisqually-1 is the first model woody plant with an available well-annotated genome. Nevertheless, a simple and rapid transformation of Nisqually-1 remains to be established. Here, we developed a novel shoot regeneration method for Nisqually-1 using leaf petiole and stem segment explants. Numerous shoots formed in the incision of explants within two weeks. The optimized shoot regeneration medium (SRM) contained 0.03 mg l<sup>-1</sup> 6-benzylaminopurine, 0.02 mg l<sup>-1</sup> indole-3-butyric acid and 0.0008 mg l<sup>-1</sup> thidiazuron. Based on this, Agrobacterium-mediated genetic transformation of stem explants was examined using the vector pBI<sub>121</sub> that contains the β-glucuronidase (GUS) as a reporter gene. Consequently, factors affecting transformation frequency of ","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Jun","modification":"2025-06-01T02:15:54.65Z","creation":"2025-06-01T02:15:54.65Z"},"accession":"S-EPMC5453977","cross_references":{"pubmed":["28572673"],"doi":["10.1038/s41598-017-02651-x"]}}