biostudies-literatureUnknown7(1)Li SGenotype Nisqually-1 is the first model woody plant with an available well-annotated genome. Nevertheless, a simple and rapid transformation of Nisqually-1 remains to be established. Here, we developed a novel shoot regeneration method for Nisqually-1 using leaf petiole and stem segment explants. Numerous shoots formed in the incision of explants within two weeks. The optimized shoot regeneration medium (SRM) contained 0.03 mg l^-1 6-benzylaminopurine, 0.02 mg l^-1 indole-3-butyric acid and 0.0008 mg l^-1 thidiazuron. Based on this, Agrobacterium-mediated genetic transformation of stem explants was examined using the vector pBI₁₂₁ that contains the β-glucuronidase (GUS) as a reporter gene. Consequently, factors affecting transformation frequency of Scientific reports2638https://www.ebi.ac.uk/biostudies/studies/S-EPMC5453977biostudies-literatureSimple, rapid and efficient transformation of genotype Nisqually-1: a basic tool for the first sequenced model tree.PMC5453977Li SWang CZhen CXu WCheng YfalseSimple, rapid and efficient transformation of genotype Nisqually-1: a basic tool for the first sequenced model tree.Genotype Nisqually-1 is the first model woody plant with an available well-annotated genome. Nevertheless, a simple and rapid transformation of Nisqually-1 remains to be established. Here, we developed a novel shoot regeneration method for Nisqually-1 using leaf petiole and stem segment explants. Numerous shoots formed in the incision of explants within two weeks. The optimized shoot regeneration medium (SRM) contained 0.03 mg l^-1 6-benzylaminopurine, 0.02 mg l^-1 indole-3-butyric acid and 0.0008 mg l^-1 thidiazuron. Based on this, Agrobacterium-mediated genetic transformation of stem explants was examined using the vector pBI₁₂₁ that contains the β-glucuronidase (GUS) as a reporter gene. Consequently, factors affecting transformation frequency of 2017-01-01T00:00:00Z2017 Jun2025-06-01T02:15:54.65Z2025-06-01T02:15:54.65ZS-EPMC54539772857267310.1038/s41598-017-02651-x