{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["102(4)"],"submitter":["Supatto W"],"pubmed_abstract":["The complex biomechanical events associated with embryo development are investigated in vivo, by using femtosecond laser pulse-induced ablation combined with multimodal nonlinear microscopy. We demonstrate controlled intravital ablations preserving local cytoskeleton dynamics and resulting in the modulation of specific morphogenetic movements in nonmutant Drosophila embryos. A quantitative description of complex movements is obtained both in GFP-expressing systems by using whole-embryo two-photon microscopy and in unlabeled nontransgenic embryos by using third harmonic generation microscopy. This methodology provides insight into the issue of mechano-sensitive gene expression by revealing the correlation of in vivo tissue deformation patterns with Twist protein expression in stomodeal cells at gastrulation."],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pagination":["1047-52"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC545833"],"repository":["biostudies-literature"],"pubmed_title":["In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses."],"pmcid":["PMC545833"],"pubmed_authors":["Supatto W","Debarre D","Beaurepaire E","Moulia B","Brouzes E","Farge E","Martin JL"],"additional_accession":[]},"is_claimable":false,"name":"In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses.","description":"The complex biomechanical events associated with embryo development are investigated in vivo, by using femtosecond laser pulse-induced ablation combined with multimodal nonlinear microscopy. We demonstrate controlled intravital ablations preserving local cytoskeleton dynamics and resulting in the modulation of specific morphogenetic movements in nonmutant Drosophila embryos. A quantitative description of complex movements is obtained both in GFP-expressing systems by using whole-embryo two-photon microscopy and in unlabeled nontransgenic embryos by using third harmonic generation microscopy. This methodology provides insight into the issue of mechano-sensitive gene expression by revealing the correlation of in vivo tissue deformation patterns with Twist protein expression in stomodeal cells at gastrulation.","dates":{"release":"2005-01-01T00:00:00Z","publication":"2005 Jan","modification":"2025-04-20T01:38:36.737Z","creation":"2019-03-27T01:08:32Z"},"accession":"S-EPMC545833","cross_references":{"pubmed":["15657140"],"doi":["10.1073/pnas.0405316102"]}}