biostudies-literatureUnknown102(4)Supatto WThe complex biomechanical events associated with embryo development are investigated in vivo, by using femtosecond laser pulse-induced ablation combined with multimodal nonlinear microscopy. We demonstrate controlled intravital ablations preserving local cytoskeleton dynamics and resulting in the modulation of specific morphogenetic movements in nonmutant Drosophila embryos. A quantitative description of complex movements is obtained both in GFP-expressing systems by using whole-embryo two-photon microscopy and in unlabeled nontransgenic embryos by using third harmonic generation microscopy. This methodology provides insight into the issue of mechano-sensitive gene expression by revealing the correlation of in vivo tissue deformation patterns with Twist protein expression in stomodeal cells at gastrulation.Proceedings of the National Academy of Sciences of the United States of America1047-52https://www.ebi.ac.uk/biostudies/studies/S-EPMC545833biostudies-literatureIn vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses.PMC545833Supatto WDebarre DBeaurepaire EMoulia BBrouzes EFarge EMartin JLfalseIn vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses.The complex biomechanical events associated with embryo development are investigated in vivo, by using femtosecond laser pulse-induced ablation combined with multimodal nonlinear microscopy. We demonstrate controlled intravital ablations preserving local cytoskeleton dynamics and resulting in the modulation of specific morphogenetic movements in nonmutant Drosophila embryos. A quantitative description of complex movements is obtained both in GFP-expressing systems by using whole-embryo two-photon microscopy and in unlabeled nontransgenic embryos by using third harmonic generation microscopy. This methodology provides insight into the issue of mechano-sensitive gene expression by revealing the correlation of in vivo tissue deformation patterns with Twist protein expression in stomodeal cells at gastrulation.2005-01-01T00:00:00Z2005 Jan2025-04-20T01:38:36.737Z2019-03-27T01:08:32ZS-EPMC5458331565714010.1073/pnas.0405316102