{"database":"biostudies-literature","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":55,"searchCount":0},"additional":{"submitter":["He J"],"funding":["NIDCR NIH HHS"],"pagination":["1036"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5462986"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8"],"pubmed_abstract":["Early childhood caries (ECC), which can lead to rampant tooth-decay that is painful and costly to treat, is one of the most prevalent infectious diseases affecting children worldwide. Previous studies support that interactions between <i>Streptococcus mutans</i> and <i>Candida albicans</i> are associated with the pathogenesis of ECC. The presence of <i>Candida</i> enhances <i>S. mutans</i> growth, fitness and accumulation within biofilms <i>in vitro</i>, although the molecular basis for these behaviors is undefined. Using an established co-cultivation biofilm model and RNA-Seq, we investigated how <i>C. albicans</i> influences the transcriptome of <i>S. mutans</i>. The presence of <i>C. albicans</i> dramatically altered gene expression in <i>S. mutans</i> in the dual-species biofilm, resulting in 393 genes differentially expressed, compared to mono-species biofilms of <i>S. mutans</i>. By Gene Ontology analysis, the majority of up-regulated genes were related to carbohydrate transport and metabolic/catabolic processes. KEGG pathway impact analysis showed elevated pyruvate and galactose metabolism, suggesting that co-cultivation with <i>C. albicans</i> influences carbohydrate utilization by <i>S. mutans</i>. Analysis of metabolites confirmed the increases in carbohydrate metabolism, with elevated amounts of formate in the culture medium of co-cultured biofilms. Moreover, co-cultivation with <i>C. albicans</i> altered transcription of <i>S. mutans</i> signal transduction (<i>comC</i> and <i>ciaRH</i>) genes associated with fitness and virulence. Interestingly, the expression of genes for mutacins (bacteriocins) and CRISPR were down-regulated. Collectively, the data provide a comprehensive insight into <i>S. mutans</i> transcriptomic changes induced by <i>C. albicans</i>, and offer novel insights into how bacterial-fungal interactions may enhance the severity of dental caries."],"journal":["Frontiers in microbiology"],"pubmed_title":["RNA-Seq Reveals Enhanced Sugar Metabolism in <i>Streptococcus mutans</i> Co-cultured with <i>Candida albicans</i> within Mixed-Species Biofilms."],"pmcid":["PMC5462986"],"funding_grant_id":["R01 DE018023","R01 DE025220"],"pubmed_authors":["Kim D","Burne RA","Richards VP","He J","Ahn SJ","Koo H","Zhou X"],"view_count":["55"],"additional_accession":[]},"is_claimable":false,"name":"RNA-Seq Reveals Enhanced Sugar Metabolism in <i>Streptococcus mutans</i> Co-cultured with <i>Candida albicans</i> within Mixed-Species Biofilms.","description":"Early childhood caries (ECC), which can lead to rampant tooth-decay that is painful and costly to treat, is one of the most prevalent infectious diseases affecting children worldwide. Previous studies support that interactions between <i>Streptococcus mutans</i> and <i>Candida albicans</i> are associated with the pathogenesis of ECC. The presence of <i>Candida</i> enhances <i>S. mutans</i> growth, fitness and accumulation within biofilms <i>in vitro</i>, although the molecular basis for these behaviors is undefined. Using an established co-cultivation biofilm model and RNA-Seq, we investigated how <i>C. albicans</i> influences the transcriptome of <i>S. mutans</i>. The presence of <i>C. albicans</i> dramatically altered gene expression in <i>S. mutans</i> in the dual-species biofilm, resulting in 393 genes differentially expressed, compared to mono-species biofilms of <i>S. mutans</i>. By Gene Ontology analysis, the majority of up-regulated genes were related to carbohydrate transport and metabolic/catabolic processes. KEGG pathway impact analysis showed elevated pyruvate and galactose metabolism, suggesting that co-cultivation with <i>C. albicans</i> influences carbohydrate utilization by <i>S. mutans</i>. Analysis of metabolites confirmed the increases in carbohydrate metabolism, with elevated amounts of formate in the culture medium of co-cultured biofilms. Moreover, co-cultivation with <i>C. albicans</i> altered transcription of <i>S. mutans</i> signal transduction (<i>comC</i> and <i>ciaRH</i>) genes associated with fitness and virulence. Interestingly, the expression of genes for mutacins (bacteriocins) and CRISPR were down-regulated. Collectively, the data provide a comprehensive insight into <i>S. mutans</i> transcriptomic changes induced by <i>C. albicans</i>, and offer novel insights into how bacterial-fungal interactions may enhance the severity of dental caries.","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2024-12-03T16:50:45.411Z","creation":"2019-03-27T02:47:06Z"},"accession":"S-EPMC5462986","cross_references":{"pubmed":["28642749"],"doi":["10.3389/fmicb.2017.01036"]}}