biostudies-literatureUnknown2(12)Forcade EChronic graft-versus-host disease (cGvHD) remains a major complication of allogeneic stem cell transplantation requiring novel therapies. CD146 and CCR5 are expressed by activated T cells and associated with increased T cell migration capacity and Th17 polarization. We performed a multiparametric flow cytometry analysis in a cohort of 40 HSCT patients together with a cGvHD murine model to understand the role of CD146-expressing subsets. We observed an increased frequency of CD146+ CD4 T cells in the 20 patients with active cGvHD with enhanced RORγt expression. This Th17-prone subset was enriched for cells coexpressing CD146 and CCR5 that harbor mixed Th1/Th17 features and were more frequent in cGvHD patients. Utilizing a murine cGvHD model with bronchiolitis obliterans (BO), we observed thJCI insight92111https://www.ebi.ac.uk/biostudies/studies/S-EPMC5470889biostudies-literatureAn activated Th17-prone T cell subset involved in chronic graft-versus-host disease sensitive to pharmacological inhibition.PMC5470889MacDonald KPBlanco PLiu LBlazar BRSerody JSKoreth JEspada ELCutler CSFlynn RSoiffer RJHill GRChu HWPaz KYang JWilkes DSGartlan KRitz JBakoyannis GLobera MLi WAntin JHAmet TForcade EGriesenauer BJiang DDu JPaczesny SfalseAn activated Th17-prone T cell subset involved in chronic graft-versus-host disease sensitive to pharmacological inhibition.Chronic graft-versus-host disease (cGvHD) remains a major complication of allogeneic stem cell transplantation requiring novel therapies. CD146 and CCR5 are expressed by activated T cells and associated with increased T cell migration capacity and Th17 polarization. We performed a multiparametric flow cytometry analysis in a cohort of 40 HSCT patients together with a cGvHD murine model to understand the role of CD146-expressing subsets. We observed an increased frequency of CD146+ CD4 T cells in the 20 patients with active cGvHD with enhanced RORγt expression. This Th17-prone subset was enriched for cells coexpressing CD146 and CCR5 that harbor mixed Th1/Th17 features and were more frequent in cGvHD patients. Utilizing a murine cGvHD model with bronchiolitis obliterans (BO), we observed th2017-01-01T00:00:00Z2017 Jun2025-04-19T13:37:16.746Z2019-03-27T02:47:38ZS-EPMC54708892861479410.1172/jci.insight.92111