biostudies-literatureSerfass JMNHLBI NIH HHSNCI NIH HHSNational Institutes of Health10097-10111https://www.ebi.ac.uk/biostudies/studies/S-EPMC5473216biostudies-literatureUnknown292(24)Endocytosis, and the subsequent trafficking of endosomes, requires dynamic physical alterations in membrane shape that are mediated in part by endophilin proteins. The endophilin B family of proteins contains an N-terminal Bin/amphiphysin/Rvs (N-BAR) domain that induces membrane curvature to regulate intracellular membrane dynamics. Whereas endophilin B1 (SH3GLB1/Bif-1) is known to be involved in a number of cellular processes, including apoptosis, autophagy, and endocytosis, the cellular function of endophilin B2 (SH3GLB2) is not well understood. In this study, we used genetic approaches that revealed that endophilin B2 is not required for embryonic development <i>in vivo</i> but that endophilin B2 deficiency impairs endosomal trafficking <i>in vitro</i>, as evidenced by suppressed endosoThe Journal of biological chemistryEndophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection.PMC5473216HL128746R56 HL128746R01 CA129682CA129682R01 CA082197CA82197Liu YTang ZYang LSerfass JMYoung MMChroneos ZCAtkinson JMKawasawa YITsotakos NWang HGTakahashi YZhou ZfalseEndophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection.Endocytosis, and the subsequent trafficking of endosomes, requires dynamic physical alterations in membrane shape that are mediated in part by endophilin proteins. The endophilin B family of proteins contains an N-terminal Bin/amphiphysin/Rvs (N-BAR) domain that induces membrane curvature to regulate intracellular membrane dynamics. Whereas endophilin B1 (SH3GLB1/Bif-1) is known to be involved in a number of cellular processes, including apoptosis, autophagy, and endocytosis, the cellular function of endophilin B2 (SH3GLB2) is not well understood. In this study, we used genetic approaches that revealed that endophilin B2 is not required for embryonic development <i>in vivo</i> but that endophilin B2 deficiency impairs endosomal trafficking <i>in vitro</i>, as evidenced by suppressed endoso2017-01-01T00:00:00Z2017 Jun2025-04-05T11:59:10.847Z2019-03-26T23:41:33ZS-EPMC54732162845544410.1074/jbc.m117.79274710.1074/jbc.M117.792747