{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Oberhofer A"],"funding":["European Research Council","Deutsche Forschungsgemeinschaft","Medical Research Council"],"pagination":["E4714-E4723"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5474823"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["114(24)"],"pubmed_abstract":["Pigment organelles, or melanosomes, are transported by kinesin, dynein, and myosin motors. As such, melanosome transport is an excellent model system to study the functional relationship between the microtubule- and actin-based transport systems. In mammalian melanocytes, it is well known that the Rab27a/melanophilin/myosin Va complex mediates actin-based transport in vivo. However, pathways that regulate the overall directionality of melanosomes on the actin/microtubule networks have not yet been delineated. Here, we investigated the role of PKA-dependent phosphorylation on the activity of the actin-based Rab27a/melanophilin/myosin Va transport complex in vitro. We found that melanophilin, specifically its C-terminal actin-binding domain (ABD), is a target of PKA. Notably, in vitro phosph"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Myosin Va's adaptor protein melanophilin enforces track selection on the microtubule and actin networks in vitro."],"pmcid":["PMC5474823"],"funding_grant_id":["G1100063","335623","SFB863"],"pubmed_authors":["Rosenfeld Y","Oberhofer A","Stepp WL","Cleetus A","Mueller-Planitz F","Spieler P","Hume AN","Okten Z"],"additional_accession":[]},"is_claimable":false,"name":"Myosin Va's adaptor protein melanophilin enforces track selection on the microtubule and actin networks in vitro.","description":"Pigment organelles, or melanosomes, are transported by kinesin, dynein, and myosin motors. As such, melanosome transport is an excellent model system to study the functional relationship between the microtubule- and actin-based transport systems. In mammalian melanocytes, it is well known that the Rab27a/melanophilin/myosin Va complex mediates actin-based transport in vivo. However, pathways that regulate the overall directionality of melanosomes on the actin/microtubule networks have not yet been delineated. Here, we investigated the role of PKA-dependent phosphorylation on the activity of the actin-based Rab27a/melanophilin/myosin Va transport complex in vitro. We found that melanophilin, specifically its C-terminal actin-binding domain (ABD), is a target of PKA. Notably, in vitro phosph","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Jun","modification":"2025-04-25T19:19:10.258Z","creation":"2019-03-27T02:47:55Z"},"accession":"S-EPMC5474823","cross_references":{"pubmed":["28559319"],"doi":["10.1073/pnas.1619473114"]}}