{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Flenker KS"],"funding":["University of Georgia Research Foundation","NIAID NIH HHS","National Institutes of Health","NIGMS NIH HHS","National Science Foundation"],"pagination":["1353-1362"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5474879"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(6)"],"pubmed_abstract":["Rapid and accurate bacterial detection methods are needed for clinical diagnostic, water, and food testing applications. The wide diversity of bacterial nucleases provides a rich source of enzymes that could be exploited as signal amplifying biomarkers to enable rapid, selective detection of bacterial species. With the exception of the use of micrococcal nuclease activity to detect Staphylococcus aureus, rapid methods that detect bacterial pathogens via their nuclease activities have not been developed. Here, we identify endonuclease I as a robust biomarker for E. coli and develop a rapid ultrasensitive assay that detects its activity. Comparison of nuclease activities of wild-type and nuclease-knockout E. coli clones revealed that endonuclease I is the predominant DNase in E. coli lysates"],"journal":["Molecular therapy : the journal of the American Society of Gene Therapy"],"pubmed_title":["Rapid Detection of Urinary Tract Infections via Bacterial Nuclease Activity."],"pmcid":["PMC5474879"],"funding_grant_id":["AI106738","R01 AI106738","T32 GM007337","R21 AI101391","1452411","AI101391"],"pubmed_authors":["Burghardt EL","Musselman CA","Kim H","McNamara JO","Owczarzy R","Huang L","Flenker KS","Kenkel EJ","Mills J","Behlke MA","Grover JM","Dutta N","Ford B","Burns WJ","Weaver TM"],"additional_accession":[]},"is_claimable":false,"name":"Rapid Detection of Urinary Tract Infections via Bacterial Nuclease Activity.","description":"Rapid and accurate bacterial detection methods are needed for clinical diagnostic, water, and food testing applications. The wide diversity of bacterial nucleases provides a rich source of enzymes that could be exploited as signal amplifying biomarkers to enable rapid, selective detection of bacterial species. With the exception of the use of micrococcal nuclease activity to detect Staphylococcus aureus, rapid methods that detect bacterial pathogens via their nuclease activities have not been developed. Here, we identify endonuclease I as a robust biomarker for E. coli and develop a rapid ultrasensitive assay that detects its activity. Comparison of nuclease activities of wild-type and nuclease-knockout E. coli clones revealed that endonuclease I is the predominant DNase in E. coli lysates","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Jun","modification":"2026-05-05T14:35:24.685Z","creation":"2019-03-26T23:40:03Z"},"accession":"S-EPMC5474879","cross_references":{"pubmed":["28391960"],"doi":["10.1016/j.ymthe.2017.03.015"]}}