biostudies-literatureUnknown7(1)Perconti GCell surface expression of alpha-enolase, a glycolytic enzyme displaying moonlighting activities, has been shown to contribute to the motility and invasiveness of cancer cells through the protein non-enzymatic function of binding plasminogen and enhancing plasmin formation. Although a few recent records indicate the involvement of protein partners in the localization of alpha-enolase to the plasma membrane, the cellular mechanisms underlying surface exposure remain largely elusive. Searching for novel interactors and signalling pathways, we used low-metastatic breast cancer cells, a doxorubicin-resistant counterpart and a non-tumourigenic mammary epithelial cell line. Here, we demonstrate by a combination of experimental approaches that epidermal growth factor (EGF) exposure, like lipopolyScientific reports3841https://www.ebi.ac.uk/biostudies/studies/S-EPMC5476664biostudies-literaturePro-invasive stimuli and the interacting protein Hsp70 favour the route of alpha-enolase to the cell surface.PMC5476664Perconti GMaranto CRomancino DPRubino PGiallongo ABongiovanni AFeo SfalsePro-invasive stimuli and the interacting protein Hsp70 favour the route of alpha-enolase to the cell surface.Cell surface expression of alpha-enolase, a glycolytic enzyme displaying moonlighting activities, has been shown to contribute to the motility and invasiveness of cancer cells through the protein non-enzymatic function of binding plasminogen and enhancing plasmin formation. Although a few recent records indicate the involvement of protein partners in the localization of alpha-enolase to the plasma membrane, the cellular mechanisms underlying surface exposure remain largely elusive. Searching for novel interactors and signalling pathways, we used low-metastatic breast cancer cells, a doxorubicin-resistant counterpart and a non-tumourigenic mammary epithelial cell line. Here, we demonstrate by a combination of experimental approaches that epidermal growth factor (EGF) exposure, like lipopoly2017-01-01T00:00:00Z2017 Jun2025-04-25T19:22:16.1Z2019-03-27T02:47:58ZS-EPMC54766642863048010.1038/s41598-017-04185-8