biostudies-literatureUnknown7(1)Su CTAntibody research has traditionally focused on heavy chains, often neglecting the important complementary role of light chains in antibody formation and secretion. In the light chain, the complementarity-determining region 3 (VL-CDR3) is specifically implicated in disease states. By modulating VL-CDR3 exposure on the scaffold through deletions in the framework region 3 (VL-FWR3), we further investigated the effects on secretion in recombinant production and antigen binding kinetics. Our random deletions of two residues in the VL-FWR3 of a Trastuzumab model showed that the single deletions could impact recombinant production without significant effect on Her2 binding. When both the selected residues were deleted, antibody secretion was additively decreased, and so was Her2 binding kinetics.Scientific reports3766https://www.ebi.ac.uk/biostudies/studies/S-EPMC5476676biostudies-literatureThe role of Antibody Vκ Framework 3 region towards Antigen binding: Effects on recombinant production and Protein L binding.PMC5476676Lua WHGan SKSu CTPoh JJLing WLfalseThe role of Antibody Vκ Framework 3 region towards Antigen binding: Effects on recombinant production and Protein L binding.Antibody research has traditionally focused on heavy chains, often neglecting the important complementary role of light chains in antibody formation and secretion. In the light chain, the complementarity-determining region 3 (VL-CDR3) is specifically implicated in disease states. By modulating VL-CDR3 exposure on the scaffold through deletions in the framework region 3 (VL-FWR3), we further investigated the effects on secretion in recombinant production and antigen binding kinetics. Our random deletions of two residues in the VL-FWR3 of a Trastuzumab model showed that the single deletions could impact recombinant production without significant effect on Her2 binding. When both the selected residues were deleted, antibody secretion was additively decreased, and so was Her2 binding kinetics.2017-01-01T00:00:00Z2017 Jun2026-05-04T16:17:43.571Z2019-03-27T02:47:58ZS-EPMC54766762863046310.1038/s41598-017-02756-3