{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ersoy SC"],"funding":["NHLBI NIH HHS"],"pagination":["173-181"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5478264"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20"],"pubmed_abstract":["The emergence and prevalence of antibiotic-resistant bacteria are an increasing cause of death worldwide, resulting in a global 'call to action' to avoid receding into an era lacking effective antibiotics. Despite the urgency, the healthcare industry still relies on a single in vitro bioassay to determine antibiotic efficacy. This assay fails to incorporate environmental factors normally present during host-pathogen interactions in vivo that significantly impact antibiotic efficacy. Here we report that standard antimicrobial susceptibility testing (AST) failed to detect antibiotics that are in fact effective in vivo; and frequently identified antibiotics that were instead ineffective as further confirmed in mouse models of infection and sepsis. Notably, AST performed in media mimicking hos"],"journal":["EBioMedicine"],"pubmed_title":["Correcting a Fundamental Flaw in the Paradigm for Antimicrobial Susceptibility Testing."],"pmcid":["PMC5478264"],"funding_grant_id":["P01 HL131474","R01 HL125352"],"pubmed_authors":["Smith JW","House JK","Mahan MJ","Barnes L","Ersoy SC","Heithoff DM","Marth JD","Tripp GK"],"additional_accession":[]},"is_claimable":false,"name":"Correcting a Fundamental Flaw in the Paradigm for Antimicrobial Susceptibility Testing.","description":"The emergence and prevalence of antibiotic-resistant bacteria are an increasing cause of death worldwide, resulting in a global 'call to action' to avoid receding into an era lacking effective antibiotics. Despite the urgency, the healthcare industry still relies on a single in vitro bioassay to determine antibiotic efficacy. This assay fails to incorporate environmental factors normally present during host-pathogen interactions in vivo that significantly impact antibiotic efficacy. Here we report that standard antimicrobial susceptibility testing (AST) failed to detect antibiotics that are in fact effective in vivo; and frequently identified antibiotics that were instead ineffective as further confirmed in mouse models of infection and sepsis. Notably, AST performed in media mimicking hos","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Jun","modification":"2025-04-19T19:05:06.584Z","creation":"2019-03-27T02:48:05Z"},"accession":"S-EPMC5478264","cross_references":{"pubmed":["28579300"],"doi":["10.1016/j.ebiom.2017.05.026"]}}