biostudies-literatureAlmamun MBryan Thomas Campbell Foundation and National Cancer InstituteNational Cancer InstituteNCI NIH HHSNational Institutes of Health1-12https://www.ebi.ac.uk/biostudies/studies/S-EPMC5478429biostudies-literatureUnknown58(9)A complete understanding of the mechanisms involved in the development of pre-B ALL is lacking. In this study, we integrated DNA methylation data and gene expression data to elucidate the impact of aberrant intergenic DNA methylation on gene expression in pre-B ALL. We found a subset of differentially methylated intergenic loci that were associated with altered gene expression in pre-B ALL patients. Notably, 84% of these regions were also bound by transcription factors (TF) known to play roles in differentiation and B-cell development in a lymphoblastoid cell line. Further, an overall downregulation of eRNA transcripts was observed in pre-B ALL patients and these transcripts were associated with the downregulation of putative target genes involved in B-cell migration, proliferation, and apLeukemia & lymphomaInferring a role for methylation of intergenic DNA in the regulation of genes aberrantly expressed in precursor B-cell acute lymphoblastic leukemia.PMC5478429CA132784R00 CA132784Kholod OJohnson NTArthur GLAlmamun MLevinson BTStuckel AJDavis JWTaylor KHfalseInferring a role for methylation of intergenic DNA in the regulation of genes aberrantly expressed in precursor B-cell acute lymphoblastic leukemia.A complete understanding of the mechanisms involved in the development of pre-B ALL is lacking. In this study, we integrated DNA methylation data and gene expression data to elucidate the impact of aberrant intergenic DNA methylation on gene expression in pre-B ALL. We found a subset of differentially methylated intergenic loci that were associated with altered gene expression in pre-B ALL patients. Notably, 84% of these regions were also bound by transcription factors (TF) known to play roles in differentiation and B-cell development in a lymphoblastoid cell line. Further, an overall downregulation of eRNA transcripts was observed in pre-B ALL patients and these transcripts were associated with the downregulation of putative target genes involved in B-cell migration, proliferation, and ap2017-01-01T00:00:00Z2017 Sep2026-05-03T19:34:58.15Z2019-03-26T23:53:47ZS-EPMC54784292809457410.1080/10428194.2016.1272683