{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["102(5)"],"submitter":["Shu S"],"pubmed_abstract":["Blebbistatin, a cell-permeable inhibitor of class-II myosins, was developed to provide a tool for studying the biologic roles of myosin II. Consistent with this use, we find that blebbistatin inhibits three myosin II-dependent processes in Dictyostelium (growth in suspension culture, capping of Con A receptors, and development to fruiting bodies) and does not inhibit growth on plates, which does not require myosin II. As expected, macropinocytosis (myosin I-dependent), contractile vacuole activity (myosin V-dependent), and phagocytosis (myosin VII-dependent), none of which requires myosin II, are not inhibited by blebbistatin in myosin II-null cells, but, unexpectedly, blebbistatin does inhibit macropinocytosis and phagocytosis by cells expressing myosin II. Expression of catalytically ina"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pagination":["1472-7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC547870"],"repository":["biostudies-literature"],"pubmed_title":["Blebbistatin and blebbistatin-inactivated myosin II inhibit myosin II-independent processes in Dictyostelium."],"pmcid":["PMC547870"],"pubmed_authors":["Liu X","Shu S","Korn ED"],"additional_accession":[]},"is_claimable":false,"name":"Blebbistatin and blebbistatin-inactivated myosin II inhibit myosin II-independent processes in Dictyostelium.","description":"Blebbistatin, a cell-permeable inhibitor of class-II myosins, was developed to provide a tool for studying the biologic roles of myosin II. Consistent with this use, we find that blebbistatin inhibits three myosin II-dependent processes in Dictyostelium (growth in suspension culture, capping of Con A receptors, and development to fruiting bodies) and does not inhibit growth on plates, which does not require myosin II. As expected, macropinocytosis (myosin I-dependent), contractile vacuole activity (myosin V-dependent), and phagocytosis (myosin VII-dependent), none of which requires myosin II, are not inhibited by blebbistatin in myosin II-null cells, but, unexpectedly, blebbistatin does inhibit macropinocytosis and phagocytosis by cells expressing myosin II. Expression of catalytically ina","dates":{"release":"2005-01-01T00:00:00Z","publication":"2005 Feb","modification":"2026-07-15T07:05:34.699Z","creation":"2019-03-27T01:08:34Z"},"accession":"S-EPMC547870","cross_references":{"pubmed":["15671182"],"doi":["10.1073/pnas.0409528102"]}}