biostudies-literatureBonyi ENIGMS NIH HHS331-340https://www.ebi.ac.uk/biostudies/studies/S-EPMC5479633biostudies-literatureUnknown154Physical stability of metal nanoparticle films on planar surfaces can be increased by employing surface modification techniques and/or type of metal nanoparticles. Subsequently, the enzymatic response of colorimetric bioassays can be increased for improved dynamic range for the detection of biomolecules. Using a model bioassay b-BSA, three planar platforms (1) poly (methyl methacrylate) (PMMA) with silver thin films (STFs), (2) silver nanowires (Ag NWs) on paper and (3) indium tin oxide (ITO) on polyethylene terephthalate (PET) were evaluated to investigate the extent of increase in the colorimetric signal. Bioassays for b-BSA and Ki-67 antigen (a real-life bioassay) in buffer were performed using microwave heating (total assay time is 25-30min) and at room temperature (a control experimenColloids and surfaces. B, BiointerfacesMetal oxide surfaces for enhanced colorimetric response in bioassays.PMC5479633UL1 GM118973Aslan KKukoyi ZUnalan HEBoone-Kukoyi ZCoskun SDaodu OBonyi EfalseMetal oxide surfaces for enhanced colorimetric response in bioassays.Physical stability of metal nanoparticle films on planar surfaces can be increased by employing surface modification techniques and/or type of metal nanoparticles. Subsequently, the enzymatic response of colorimetric bioassays can be increased for improved dynamic range for the detection of biomolecules. Using a model bioassay b-BSA, three planar platforms (1) poly (methyl methacrylate) (PMMA) with silver thin films (STFs), (2) silver nanowires (Ag NWs) on paper and (3) indium tin oxide (ITO) on polyethylene terephthalate (PET) were evaluated to investigate the extent of increase in the colorimetric signal. Bioassays for b-BSA and Ki-67 antigen (a real-life bioassay) in buffer were performed using microwave heating (total assay time is 25-30min) and at room temperature (a control experimen2017-01-01T00:00:00Z2017 Jun2025-04-05T14:23:27.81Z2019-03-26T23:38:49ZS-EPMC54796332836472010.1016/j.colsurfb.2017.03.030