biostudies-literatureDamase TRNIGMS NIH HHS4081https://www.ebi.ac.uk/biostudies/studies/S-EPMC5481426biostudies-literatureUnknown7(1)We report the release of catalytic DNA walkers from hydrogel microparticles and the detection of those walkers by substrate-coated microparticles. This might be considered a synthetic biology analog of molecular signal release and reception. One type of particles was coated with components of a DNA one-step strand displacement (OSD) reaction to release the walker. A second type of particle was coated with substrate (or "track") for the molecular walker. We distinguish these particle types using fluorescence barcoding: we synthesized and distinguished multiple particle types with multicolor fluorescence microscopy and automated image analysis software. This represents a step toward amplified, multiplex, and microscopically localized detection based on DNA nanotechnology.Scientific reportsBiomimetic Molecular Signaling using DNA Walkers on Microparticles.PMC5481426P20 GM103408Samuel BAllen PBDamase TRSpencer AfalseBiomimetic Molecular Signaling using DNA Walkers on Microparticles.We report the release of catalytic DNA walkers from hydrogel microparticles and the detection of those walkers by substrate-coated microparticles. This might be considered a synthetic biology analog of molecular signal release and reception. One type of particles was coated with components of a DNA one-step strand displacement (OSD) reaction to release the walker. A second type of particle was coated with substrate (or "track") for the molecular walker. We distinguish these particle types using fluorescence barcoding: we synthesized and distinguished multiple particle types with multicolor fluorescence microscopy and automated image analysis software. This represents a step toward amplified, multiplex, and microscopically localized detection based on DNA nanotechnology.2017-01-01T00:00:00Z2017 Jun2024-11-09T08:04:43.205Z2019-03-27T02:48:18ZS-EPMC54814262864248810.1038/s41598-017-04316-1