biostudies-literatureUnknown116(10)Wozny AS<h4>Background</h4>Head and neck squamous cell carcinoma (HNSCC) are resistant to standard treatments, partly due to cancer stem cells (CSCs) localised in hypoxic niches. Compared to X-rays, carbon ion irradiation relies on better ballistic properties, higher relative biological effectiveness and the absence of oxygen effect. Hypoxia-inducible factor-1α (HIF-1α) is involved in the resistance to photons, whereas its role in response to carbon ions remains unclear.<h4>Methods</h4>Two HNSCC cell lines and their CSC sub-population were studied in response to photons or carbon ion irradiation, in normoxia or hypoxia, after inhibition or not of HIF-1α.<h4>Results</h4>Under hypoxia, compared to non-CSCs, HIF-1α is expressed earlier in CSCs. A combined effect photons/hypoxia, less observed with caBritish journal of cancer1340-1349https://www.ebi.ac.uk/biostudies/studies/S-EPMC5482725biostudies-literatureDifferential pattern of HIF-1α expression in HNSCC cancer stem cells after carbon ion or photon irradiation: one molecular explanation of the oxygen effect.PMC5482725Lalle PCunha MAlphonse GSaintigny YArdail DWozny ASBattiston-Montagne PLauret ABeuve MBlond EMagne NGuy JBRodriguez-Lafrasse CfalseDifferential pattern of HIF-1α expression in HNSCC cancer stem cells after carbon ion or photon irradiation: one molecular explanation of the oxygen effect.<h4>Background</h4>Head and neck squamous cell carcinoma (HNSCC) are resistant to standard treatments, partly due to cancer stem cells (CSCs) localised in hypoxic niches. Compared to X-rays, carbon ion irradiation relies on better ballistic properties, higher relative biological effectiveness and the absence of oxygen effect. Hypoxia-inducible factor-1α (HIF-1α) is involved in the resistance to photons, whereas its role in response to carbon ions remains unclear.<h4>Methods</h4>Two HNSCC cell lines and their CSC sub-population were studied in response to photons or carbon ion irradiation, in normoxia or hypoxia, after inhibition or not of HIF-1α.<h4>Results</h4>Under hypoxia, compared to non-CSCs, HIF-1α is expressed earlier in CSCs. A combined effect photons/hypoxia, less observed with ca2017-01-01T00:00:00Z2017 May2025-06-01T12:11:07.488Z2025-06-01T12:11:07.488ZS-EPMC54827252840765310.1038/bjc.2017.100