{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["232(9)"],"submitter":["Garcia A"],"funding":["Canada Foundation for Innovation"],"pubmed_abstract":["The advent of super-resolution microscopy allowed for new insights into cellular and physiological processes of normal and diseased cells. In this study, we report for the first time on the super-resolved DNA structure of buccal cells from patients with Alzheimer's disease (AD) versus age- and gender-matched healthy, non-caregiver controls. In this super-resolution study cohort of 74 participants, buccal cells were collected and their spatial DNA organization in the nucleus examined by 3D Structured Illumination Microscopy (3D-SIM). Quantitation of the super-resolution DNA structure revealed that the nuclear super-resolution DNA structure of individuals with AD significantly differs from that of their controls (p < 0.05) with an overall increase in the measured DNA-free/poor spaces. This represents a significant increase in the interchromatin compartment. We also find that the DNA structure of AD significantly differs in mild, moderate, and severe disease with respect to the DNA-containing and DNA-free/poor spaces. We conclude that whole genome remodeling is a feature of buccal cells in AD."],"journal":["Journal of cellular physiology"],"pagination":["2387-2395"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5485033"],"repository":["biostudies-literature"],"pubmed_title":["Super-resolution structure of DNA significantly differs in buccal cells of controls and Alzheimer's patients."],"pmcid":["PMC5485033"],"pubmed_authors":["Mathur S","Anderson J","Mai S","Huang D","Itorralba J","Kalaw MC","Garcia A","McAvoy E","Luedke A","Righolt C","Righolt A"],"additional_accession":[]},"is_claimable":false,"name":"Super-resolution structure of DNA significantly differs in buccal cells of controls and Alzheimer's patients.","description":"The advent of super-resolution microscopy allowed for new insights into cellular and physiological processes of normal and diseased cells. In this study, we report for the first time on the super-resolved DNA structure of buccal cells from patients with Alzheimer's disease (AD) versus age- and gender-matched healthy, non-caregiver controls. In this super-resolution study cohort of 74 participants, buccal cells were collected and their spatial DNA organization in the nucleus examined by 3D Structured Illumination Microscopy (3D-SIM). Quantitation of the super-resolution DNA structure revealed that the nuclear super-resolution DNA structure of individuals with AD significantly differs from that of their controls (p < 0.05) with an overall increase in the measured DNA-free/poor spaces. This represents a significant increase in the interchromatin compartment. We also find that the DNA structure of AD significantly differs in mild, moderate, and severe disease with respect to the DNA-containing and DNA-free/poor spaces. We conclude that whole genome remodeling is a feature of buccal cells in AD.","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Sep","modification":"2026-03-15T21:18:01.554Z","creation":"2019-03-27T02:48:31Z"},"accession":"S-EPMC5485033","cross_references":{"pubmed":["27996096"],"doi":["10.1002/jcp.25751"]}}