biostudies-literatureBousleiman JNSFMINECO-FEDERE1133https://www.ebi.ac.uk/biostudies/studies/S-EPMC5485957biostudies-literatureUnknown18(6)A study of factors proposed to affect metallothionein-3 (MT3) function was carried out to elucidate the opaque role MT3 plays in human metalloneurochemistry. Gene expression of <i>Mt2</i> and <i>Mt3</i> was examined in tissues extracted from the dentate gyrus of mouse brains and in human neuronal cell cultures. The whole-genome gene expression analysis identified significant variations in the mRNA levels of genes associated with zinc homeostasis, including <i>Mt2</i> and <i>Mt3</i>. <i>Mt3</i> was found to be the most differentially expressed gene in the identified groups, pointing to the existence of a factor, not yet identified, that differentially controls <i>Mt3</i> expression. To examine the expression of the human metallothioneins in neurons, mRNA levels of <i>MT3</i> and <i>MT2</i> International journal of molecular sciencesFunction of Metallothionein-3 in Neuronal Cells: Do Metal Ions Alter Expression Levels of MT3?PMC5485957CHE-1151957BIO2015-67358-C2-2-PAustin RNKi SKalachikov SWiqas ASever MSu APinsky ASilver RMorozova IBousleiman JfalseFunction of Metallothionein-3 in Neuronal Cells: Do Metal Ions Alter Expression Levels of MT3?A study of factors proposed to affect metallothionein-3 (MT3) function was carried out to elucidate the opaque role MT3 plays in human metalloneurochemistry. Gene expression of <i>Mt2</i> and <i>Mt3</i> was examined in tissues extracted from the dentate gyrus of mouse brains and in human neuronal cell cultures. The whole-genome gene expression analysis identified significant variations in the mRNA levels of genes associated with zinc homeostasis, including <i>Mt2</i> and <i>Mt3</i>. <i>Mt3</i> was found to be the most differentially expressed gene in the identified groups, pointing to the existence of a factor, not yet identified, that differentially controls <i>Mt3</i> expression. To examine the expression of the human metallothioneins in neurons, mRNA levels of <i>MT3</i> and <i>MT2</i> 2017-01-01T00:00:00Z2017 May2026-04-14T09:09:33.219Z2019-03-27T02:48:35ZS-EPMC54859572858709810.3390/ijms18061133