biostudies-literatureUnknown7Cheng CMicrobes employ the thioredoxin system to defend against oxidative stress and ensure correct disulfide bonding to maintain protein function. <i>Listeria monocytogenes</i> has been shown to encode a putative thioredoxin, TrxA, but its biological roles and underlying mechanisms remain unknown. Here, we showed that expression of <i>L. monocytogenes</i> TrxA is significantly induced in bacteria treated with the thiol-specific oxidizing agent, diamide. Deletion of <i>trxA</i> markedly compromised tolerance of the pathogen to diamide, and mainly impaired early stages of infection in human intestinal epithelial Caco-2 cells. In addition, most <i>trxA</i> mutant bacteria were not associated with polymerized actin, and the rare bacteria that were associated with polymerized actin displayed very shoFrontiers in cellular and infection microbiology287https://www.ebi.ac.uk/biostudies/studies/S-EPMC5487381biostudies-literatureThioredoxin A Is Essential for Motility and Contributes to Host Infection of <i>Listeria monocytogenes</i> via Redox Interactions.PMC5487381Cheng CJiang LFreitag NEDong ZHuang HHan XWang HMa TSong HShao CYang YChen ZFang WWang XSun JfalseThioredoxin A Is Essential for Motility and Contributes to Host Infection of <i>Listeria monocytogenes</i> via Redox Interactions.Microbes employ the thioredoxin system to defend against oxidative stress and ensure correct disulfide bonding to maintain protein function. <i>Listeria monocytogenes</i> has been shown to encode a putative thioredoxin, TrxA, but its biological roles and underlying mechanisms remain unknown. Here, we showed that expression of <i>L. monocytogenes</i> TrxA is significantly induced in bacteria treated with the thiol-specific oxidizing agent, diamide. Deletion of <i>trxA</i> markedly compromised tolerance of the pathogen to diamide, and mainly impaired early stages of infection in human intestinal epithelial Caco-2 cells. In addition, most <i>trxA</i> mutant bacteria were not associated with polymerized actin, and the rare bacteria that were associated with polymerized actin displayed very sho2017-01-01T00:00:00Z20172025-05-29T19:05:06.274Z2025-05-29T19:05:06.274ZS-EPMC54873812870237810.3389/fcimb.2017.00287