biostudies-literatureGavrilov SRussian Foundation for Basic ResearchRussian Science Foundation1228https://www.ebi.ac.uk/biostudies/studies/S-EPMC5492636biostudies-literatureUnknown8<i>Melioribacter roseus</i>, a representative of recently proposed Ignavibacteriae phylum, is a metabolically versatile thermophilic bacterium, inhabiting subsurface biosphere of the West-Siberian megabasin and capable of growing on various substrates and electron acceptors. Genomic analysis followed by inhibitor studies and membrane potential measurements of aerobically grown <i>M. roseus</i> cells revealed the activity of aerobic respiratory electron transfer chain comprised of respiratory complexes I and IV, and an alternative complex III. Phylogeny reconstruction revealed that oxygen reductases belonged to atypical <i>cc(o/b)o₃</i> -type and canonical <i>cbb₃</i> -type cytochrome oxidases. Also, two molybdoenzymes of <i>M. roseus</i> were affiliated either with TtFrontiers in microbiologyRespiratory Pathways Reconstructed by Multi-Omics Analysis in <i>Melioribacter roseus</i>, Residing in a Deep Thermal Aquifer of the West-Siberian Megabasin.PMC549263614-24-0016514-24-0015914-50-0002917-04-02173Podosokorskaya OMuntyan MMerkel AGavrilov SAltukhov IGovorun VAlexeev DKublanov IKhomyakova MBonch-Osmolovskaya EButenko IfalseRespiratory Pathways Reconstructed by Multi-Omics Analysis in <i>Melioribacter roseus</i>, Residing in a Deep Thermal Aquifer of the West-Siberian Megabasin.<i>Melioribacter roseus</i>, a representative of recently proposed Ignavibacteriae phylum, is a metabolically versatile thermophilic bacterium, inhabiting subsurface biosphere of the West-Siberian megabasin and capable of growing on various substrates and electron acceptors. Genomic analysis followed by inhibitor studies and membrane potential measurements of aerobically grown <i>M. roseus</i> cells revealed the activity of aerobic respiratory electron transfer chain comprised of respiratory complexes I and IV, and an alternative complex III. Phylogeny reconstruction revealed that oxygen reductases belonged to atypical <i>cc(o/b)o₃</i> -type and canonical <i>cbb₃</i> -type cytochrome oxidases. Also, two molybdoenzymes of <i>M. roseus</i> were affiliated either with Tt2017-01-01T00:00:00Z20172026-05-04T17:12:36.322Z2019-03-27T02:49:00ZS-EPMC54926362871335510.3389/fmicb.2017.01228