{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6(1)"],"submitter":["Liu Y"],"pubmed_abstract":["Characterization of transcription factor-DNA interaction is of high importance in elucidating the molecular mechanisms of gene transcriptions. DNA-based affinity probes were developed to capture and identify transcription factors by covalent crosslinking; however, the requirement of a crosslinker on the affinity probe remains a disadvantage, as the crosslinker itself often interferes with the protein-DNA interactions. We report a dual-probe method able to capture DNA-binding transcription factors with unmodified protein-binding sites in scenarios where conventional probes have failed. We have also shown the method's converse application in selecting specific transcription factor-binding DNA sequences from a probe library and its extension to studying proteins recognizing epigenetic marks. This study may provide a new tool for exploring DNA-binding proteins in biology."],"journal":["Chemical science"],"pagination":["745-751"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5494549"],"repository":["biostudies-literature"],"pubmed_title":["Photoaffinity labeling of transcription factors by DNA-templated crosslinking."],"pmcid":["PMC5494549"],"pubmed_authors":["Zhang W","Liu Y","Chen N","Li X","Zheng H","Chen L","Chen X","Zheng W","Zhou X"],"additional_accession":[]},"is_claimable":false,"name":"Photoaffinity labeling of transcription factors by DNA-templated crosslinking.","description":"Characterization of transcription factor-DNA interaction is of high importance in elucidating the molecular mechanisms of gene transcriptions. DNA-based affinity probes were developed to capture and identify transcription factors by covalent crosslinking; however, the requirement of a crosslinker on the affinity probe remains a disadvantage, as the crosslinker itself often interferes with the protein-DNA interactions. We report a dual-probe method able to capture DNA-binding transcription factors with unmodified protein-binding sites in scenarios where conventional probes have failed. We have also shown the method's converse application in selecting specific transcription factor-binding DNA sequences from a probe library and its extension to studying proteins recognizing epigenetic marks. This study may provide a new tool for exploring DNA-binding proteins in biology.","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Jan","modification":"2021-02-21T10:52:28Z","creation":"2019-03-27T02:49:09Z"},"accession":"S-EPMC5494549","cross_references":{"pubmed":["28706637"],"doi":["10.1039/c4sc01953a"]}}