{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["45(11)"],"submitter":["Lee HC"],"pubmed_abstract":["RNA-binding proteins (RBPs) are involved in mRNA splicing, maturation, transport, translation, storage and turnover. Here, we identified ACOT7 mRNA as a novel target of human WIG1. ACOT7 mRNA decay was triggered by the microRNA miR-9 in a WIG1-dependent manner via classic recruitment of Argonaute 2 (AGO2). Interestingly, AGO2 was also recruited to ACOT7 mRNA in a WIG1-dependent manner in the absence of miR-9, which indicates an alternative model whereby WIG1 controls AGO2-mediated gene silencing. The WIG1-AGO2 complex attenuated translation initiation via an interaction with translation initiation factor 5B (eIF5B). These results were confirmed using a WIG1 tethering system based on the MS2 bacteriophage coat protein and a reporter construct containing an MS2-binding site, and by immunopre"],"journal":["Nucleic acids research"],"pagination":["6894-6910"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5499809"],"repository":["biostudies-literature"],"pubmed_title":["WIG1 is crucial for AGO2-mediated ACOT7 mRNA silencing via miRNA-dependent and -independent mechanisms."],"pmcid":["PMC5499809"],"pubmed_authors":["Jung SH","Lee HC","Han K","Gorospe M","Park HJ","Hwang HJ","Martindale JL","Dudekula DB","Lee JS","De S","Kang D","Jeong S","Lee EK","Ko YG","Park B","Park SK","Lee JH"],"additional_accession":[]},"is_claimable":false,"name":"WIG1 is crucial for AGO2-mediated ACOT7 mRNA silencing via miRNA-dependent and -independent mechanisms.","description":"RNA-binding proteins (RBPs) are involved in mRNA splicing, maturation, transport, translation, storage and turnover. Here, we identified ACOT7 mRNA as a novel target of human WIG1. ACOT7 mRNA decay was triggered by the microRNA miR-9 in a WIG1-dependent manner via classic recruitment of Argonaute 2 (AGO2). Interestingly, AGO2 was also recruited to ACOT7 mRNA in a WIG1-dependent manner in the absence of miR-9, which indicates an alternative model whereby WIG1 controls AGO2-mediated gene silencing. The WIG1-AGO2 complex attenuated translation initiation via an interaction with translation initiation factor 5B (eIF5B). These results were confirmed using a WIG1 tethering system based on the MS2 bacteriophage coat protein and a reporter construct containing an MS2-binding site, and by immunopre","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Jun","modification":"2026-05-05T17:15:44.111Z","creation":"2019-03-27T02:49:38Z"},"accession":"S-EPMC5499809","cross_references":{"pubmed":["28472401"],"doi":["10.1093/nar/gkx307"]}}